Point-of-Care System for HTLV-1 Proviral Load Quantification by Digital Mediator Displacement LAMP
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Cultures, Viral Strains, and Sample Material
2.2. dLAMP: Oligonucleotides and LAMP Reaction
2.3. Functional Model of the POCT Instrument
2.4. LabDisk Cartridge Manufacturing Including Reagent Pre-Storage
2.5. LabDisk Cartridge Automation Principle
3. Results and Discussions
3.1. Quantification of Nucleic Acids per MT-2 Cell with Fresh and Dried Reagents
3.2. Efficiency of Automated Nucleic Acid Extraction and Purification on LabDisk
3.3. Sample-to-Answer Testing with HTLV-1-Infected Lymphocytes
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Becherer, L.; Hess, J.F.; Frischmann, S.; Bakheit, M.; Nitschko, H.; Stinco, S.; Zitz, F.; Hofer, H.; Porro, G.; Hausladen, F.; et al. Point-of-Care System for HTLV-1 Proviral Load Quantification by Digital Mediator Displacement LAMP. Micromachines 2021, 12, 159. https://doi.org/10.3390/mi12020159
Becherer L, Hess JF, Frischmann S, Bakheit M, Nitschko H, Stinco S, Zitz F, Hofer H, Porro G, Hausladen F, et al. Point-of-Care System for HTLV-1 Proviral Load Quantification by Digital Mediator Displacement LAMP. Micromachines. 2021; 12(2):159. https://doi.org/10.3390/mi12020159
Chicago/Turabian StyleBecherer, Lisa, Jacob Friedrich Hess, Sieghard Frischmann, Mohammed Bakheit, Hans Nitschko, Silvina Stinco, Friedrich Zitz, Hannes Hofer, Giampiero Porro, Florian Hausladen, and et al. 2021. "Point-of-Care System for HTLV-1 Proviral Load Quantification by Digital Mediator Displacement LAMP" Micromachines 12, no. 2: 159. https://doi.org/10.3390/mi12020159