Microfluidic Formation of Double-Stacked Planar Bilayer Lipid Membranes by Controlling the Water-Oil Interface
Abstract
:1. Introduction
2. Materials and Methods
2.1. Regents and Chemicals
2.2. Concept of Double-Stacked pBLM Formation Using Five-Layered Microchannels
2.3. Hydrodynamic Simulation of Five-Layered Microchannels
2.4. Fabrication of Five-Layered Microchannels
2.5. Microfluidic Experiment of Double-Stacked pBLM Formation
2.6. Reconstitution of Membrane Protein to the Double-Stacked pBLMs
3. Results and Discussion
3.1. Hydrodynamic Simulation for Designing the Double-Stacked BLMs
3.2. Formation of Double-Stacked pBLMs Using Five-Layered Microchannels
3.3. Channel Current Measurement of Nanopores Reconstituted in the Double-Stacked BLMs
4. Conclusions
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Shoji, K.; Kawano, R. Microfluidic Formation of Double-Stacked Planar Bilayer Lipid Membranes by Controlling the Water-Oil Interface. Micromachines 2018, 9, 253. https://doi.org/10.3390/mi9050253
Shoji K, Kawano R. Microfluidic Formation of Double-Stacked Planar Bilayer Lipid Membranes by Controlling the Water-Oil Interface. Micromachines. 2018; 9(5):253. https://doi.org/10.3390/mi9050253
Chicago/Turabian StyleShoji, Kan, and Ryuji Kawano. 2018. "Microfluidic Formation of Double-Stacked Planar Bilayer Lipid Membranes by Controlling the Water-Oil Interface" Micromachines 9, no. 5: 253. https://doi.org/10.3390/mi9050253