The real-time detection of banana bunches and stalks in banana orchards is a key technology in the application of agricultural robots. The complex conditions of the orchard make accurate detection a difficult task, and the light weight of the deep learning network is an application trend. This study proposes and compares two improved YOLOv4 neural network detection models in a banana orchard. One is the YOLO-Banana detection model, which analyzes banana characteristics and network structure to prune the less important network layers; the other is the YOLO-Banana-l4 detection model, which, by adding a YOLO head layer to the pruned network structure, explores the impact of a four-scale prediction structure on the pruning network. The results show that YOLO-Banana and YOLO-Banana-l4 could reduce the network weight and shorten the detection time compared with YOLOv4. Furthermore, YOLO-Banana detection model has the best performance, with good detection accuracy for banana bunches and stalks in the natural environment. The average precision (AP) values of the YOLO-Banana detection model on banana bunches and stalks are 98.4% and 85.98%, and the mean average precision (mAP) of the detection model is 92.19%. The model weight is reduced from 244 to 137 MB, and the detection time is shortened from 44.96 to 35.33 ms. In short, the network is lightweight and has good real-time performance and application prospects in intelligent management and automatic harvesting in the banana orchard. Full article

The wheat plants were pretreated with the selective herbicide Serrate^® (Syngenta) and subsequently subjected to drought or flooding stress for 7 days. The gas exchange parameters, chlorophyll a fluorescence and leaf pigment content were measured. The measurements were performed during the stress period and after 4 days of plants recovery. Herbicide pretreatment did not cause significant alterations in photosynthesis and fluorescence parameters in alone- or combined-treated seedlings. A significant reduction in gas exchange parameters (net photosynthesis rate, stomatal conductance, transpiration rate, and water use efficiency), F_v/F_m and F_v/F₀ values during drought or flooding was observed. The disruption of photosynthesis together with reduction in the pigment content was stronger in droughted than flooded plants. When the normal irrigation was restored, the gas exchange and fluorescence parameters tended to increase. The comparative analysis of recovery and resilience indices of photosynthetic traits indicate that the plants subjected to drought recovered better than those subjected to flooding stress. Full article

The present research was undertaken to study the antifungal activities of Origanum onites L. and Ziziphora clinopodioides L. essential oils against three different isolates (M1-5, M2-1 and M3-5) of Botrytis cinerea (in vitro tests) and to investigate the vapor contact impacts on fungus and strawberry fruit quality (in vivo tests). Antifungal activities of these oils were tested by following the poisoned food technique at four different concentrations (0.25, 0.50, 1.00 and 2.00 mL/L) against B. cinerea. In vitro studies suggested that the 0.50 mL/L and 1.00 mL/L doses of O. onites and 1.00 mL/L and 2.00 mL/L doses of Z. clinopodioides provide high mycelial growth inhibition, 85.29–94.12% and 39.12–94.12%, respectively, by direct addition to food. Thus, these doses were tested in in vivo conditions, as a vapor contact treatment against two isolates (M1-5 and M3-5) of B. cinerea inoculated on strawberry cv. Camarosa fruits. Results showed that both O. onites and Z. clinopodioides essential oils have a moderate to high impact on the prevention of gray mold. The oils were also found to have a slight to moderate impact on weight loss and the loss of soluble solids concentration. Overall, the results demonstrated that the tested oils are a potential biodegradable alternative to fungicides. Full article

This work focused on the recovery and application of bioactive and functional compounds (BFC) from a waste matrix, the Opuntia ficus-indica (L.) Miller fruit peel (OFP), using different extraction methods: maceration and Extractor Naviglio^®. The presence of BFC was initially evaluated by following the extraction yields and antioxidant activity using the Folin-Ciocȃlteu reagent and the DPPH method, respectively. The quali-quantitative content of BFC was also verified with UHPLC/UV-ESI-HRMS profile of OFP extracts. The evaluation of the BFC yields is aimed at using extract components, such as betanins, in the technological application as textile dye based on natural pigments: we evaluated the stability of the extracted dyes, tested the dyeing power and assessed the stability of the dye on the textile material. The results showed different compositions depending on the polarities of the solvents. The ethanol-water mixture extracts obtained using Naviglio method revealed an important presence in terms of total phenolic compound yields. Wool, linen and cotton fibers were chosen for the dyeing tests. Rock alum and lemon juice, two etching methods, were used. Results suggest that the OFP may be of great interest as a natural source of BFC for food, nutraceutical and technological applications in the sustainability context. Full article

This study aimed to analyze the biological activity of the essential oil Pogostemon cablin (PCEO) to determine the antioxidant, antimicrobial, antibiofilm, insecticidal activity, and chemical composition of the essential oil. We analyzed the structure of biofilms on various surfaces using the MALDI-TOF MS Biotyper and evaluated the antimicrobial effect of the vapor phase of the essential oil in a food model. We determined the main volatile components of PCEO as patchouli alcohol 31.0%, α-bulnesene 21.3%, and α-guaiene 14.3%. The free radical scavenging activity was high and reached 71.4 ± 0.9%, corresponding to 732 ± 8.1 TEAC. The antimicrobial activity against bacteria was weak to moderate. We recorded strong activity against yeast. The antifungal activity was very weak in the contact application. Biofilm-producing bacteria were moderately inhibited by PCEO. The change in biofilm structure due to essential oil was demonstrated by MALDI-TOF MS Biotyper analysis. Vapor phase application in a food model showed relatively strong effects against bacteria and significantly higher antifungal efficacy. The insecticidal activity was observed only at higher concentrations of essential oil. Based on the findings, PCEO can be used in the food industry as an antifungal substance in extending the shelf life of bakery products and as protection in the storage of root vegetables. Full article

Using high-salinity water for plant fertigation may have negative consequences for plant growth, overall yield and crop quality. In the present study, the effects of NaCl-salinity in conjunction with three different ammonium to nitrate ratios (Nr) on tomato (Solanum lycopersicum Mill.) plant growth, nutritional status, yield, fruit quality and postharvest storage were examined. The electrical conductivity (EC) was increased by adding NaCl into the nutrient solution and three different Nr ratios were applied, Nr0.05, Nr0.10 and Nr0.15, while the other macronutrient and micronutrient concentrations were constant in all treatments. The EC of the nutrient solution supplied to the plants was 2.2 mS cm⁻¹ at the low salinity treatments and 7.5 mS cm⁻¹ at the high salinity treatments. Increased salinity resulted in decreased plant growth factors and fruit yield, despite the Nr. An increased Nr reduced the pH value of the nutrient solution, while the fruits of the plants cultivated under high salinity obtained reduced Ca, K, Mg, P and N content. Reduced Ca content can lead to blossom end rot disorder and this was evidenced on tomato cultivation under high salinity. However, in the present study, this disorder appeared at the very late stages and did not affect the marketability of the fruits. On the other hand, both salinity and Nr0.15 increased fruit firmness at harvest or following storage, while citric acid, total soluble solid and vitamin C contents were also increased by salinity. Total phenols were increased by salinity at harvest, and lycopene and β-carotene content were increased by salinity at harvest and/or following storage at 12 °C or 25 °C. Salinity enhances fruit quality and improves the organoleptic characteristics of the crop, while an appropriate Nr ratio may restrict the detrimental effects of salinity on the nutritional status of plants by regulating the pH in hydroponic systems. Full article

Brassinolides (BRs) are naturally-occurring phytohormones, which are essentially important to improve the crop adoptive capacity to various stresses. Spray volume (SV) and agrochemical application methods are associated with chemical deposition and field efficiency. The objective of this study was to compare the possible effects of 28-Homobrassinolide (HBL) dosages 18, 22, and 30 mg a.i. ha⁻¹ for unmanned aerial vehicle (UAV) sprayers (15 L ha⁻¹ and 30 L ha⁻¹) and 22 mg a.i. ha⁻¹ for Knapsack manual sprayers (KMS) (450 L ha⁻¹) at maize silking stage on droplets deposition distribution, photosynthetic parameters, grain filling process and yield. The results showed that the droplet deposition of UAV (15, 30 L ha⁻¹) was 47.04%, 8.89% higher than KMS. However, the UAV sprayer had a poor droplet deposition distribution. HBL significantly increased the photosynthetic parameters, grain filling rate, and yield. A UAV spray volume of 15 L ha⁻¹ with 22 mg a.i. ha⁻¹ significantly increased grains yield by 4.16–5.64%, 7.5–12.09% compared to KMS and CK in both years. Considering the high efficiency of the UAV sprayer and better effects of HBL on final yield, spraying 22~30 mg a.i. ha⁻¹ with UAV spray volume 15 L ha⁻¹ at the silking stage could be a better strategy. Full article

One important issue faced by wheat producers is temporal and spatial yield variation management at a within-field scale. Vegetation indices derived from remote-sensing platforms, such as Landsat, can provide vital information characterising this variability and allow crop yield indicators development to map productivity. However, the most appropriate vegetation index and crop growth stage for use in yield mapping is often unclear. This study considered vegetation indices and growth stages selection and built and tested models to predict within-field yield variation. We used 48 wheat yield monitor maps to build linear-mixed models for predicting yield that were tested using leave-one-field-out cross-validation. It was found that some of the simplest models were not improved upon (by more complex models) for the prediction of the spatial pattern of the high and low yielding areas (the within-field yield ranking). In addition, predictions of longer-term average yields were generally more accurate than predictions of yield for single years. Therefore, the predictions over multiple years are valuable for revealing consistent spatial patterns in yield. The results demonstrate the potential and limitations of tools based on remote-sensing data that might provide growers with better knowledge of within-field variation to make more informed management decisions. Full article

The need to supply food for a growing world population, while protecting the environment by using sustainable agriculture, presents a major challenge, in terms of decreasing the use of chemical fertilizers and pesticides. The application of Pseudozyma aphidis cells onto crop plants considerably reduced infection by several pathogens, as well as significantly enhancing plant growth and yield. It was demonstrated in the current work that P. aphidis can enhance cucumber and tomato growth, both in vitro and in the greenhouse. P. aphidis was active when applied either via spray treatment or by seed coating. A significant promotion effect of P. aphidis on tomato and cucumber growth, flowering, and tomato yield and ripening, as compared to control plants, was demonstrated. Specifically, a 10–30% increase in tomato and cucumber plant height and leaf number, a 45% increase in average fruit weight, a 120% increase in tomato fruit ripening, and a flowering time advanced by two weeks were demonstrated. In conclusion, P. aphidis can significantly enhance the growth and yield of crop plants and can, thus, be used as bio-fertilizer. Full article

The transition between the dormancy and budburst is accompanied by physiological and biochemical changes, which include variations in the carbohydrates, enzymatic activity, as well as hormones present inside the buds. The present study aimed to evaluate the biochemical changes that occur in dormant buds and forced bursting of three Vitis vinifera L. white varieties (Alvarinho, Fernão-Pires, and Loureiro) from two vineyards located in the Vinhos Verdes Demarcated Region, NW Portugal. For this purpose, the contents of malondialdehyde (MDA), proline, carbohydrates, and gibberellic acid (GA₃), as well as antioxidant enzymes activity were analysed. The results showed that the MDA, proline, total soluble sugars, and GA₃ contents tended to be higher at budburst. In contrast, the starch content was higher at dormancy. The enzymatic activities of ascorbate peroxidase (APX), peroxidase (POX), and superoxide dismutase (SOD) were lower at budburst, compared with dormancy, while the reverse was observed in catalase (CAT). Significant differences were also observed between varieties for most biochemical parameters analysed, with the exception of CAT activity. Alvarinho stood out from other varieties for presenting higher MDA, proline, soluble sugars, and GA₃ content, while Fernão-Pires displayed higher values of APX and POX activities and starch content. To our knowledge, the present work is the first attempt to examine the biochemical changes in dormant buds and bursting, which determine the vegetative and reproductive continuity of grapevines. This information contributes to better vineyard management, particularly important in light of the climate change scenarios, which can affect the biochemical mechanisms of the buds and compromise the yield and quality of grapevines. Full article

Methane (CH${}_4$) emissions from dairy husbandry are a hot topic in the context of active climate protection, where housing systems with slatted floors and slurry storage inside are in general expected to emit more than systems with solid floors. There are multiple factors, including climate conditions, that modulate the emission pattern. In this study, we investigated interrelations between CH${}_4$ emission patterns and climate conditions as well as differences between farm locations versus floor effects. We considered three data sets with 265, 264 and 275 hourly emission values from two housing systems (one slatted, one solid floor) in Switzerland and one system with solid floors in Germany. Each data set incorporated measurements in summer, winter and a transition season. The average CH${}_4$ emission was highest for the slatted floor system. For the solid floor systems, CH${}_4$ emissions at the Swiss location were around 30% higher compared to the German location. The shape of the distributions for the two solid floor systems was rather similar but very different from the distribution for the slatted floor system, which showed higher prevalence for extreme emissions. Rank correlations, which measure the degree of similarity between two rankings in terms of linear relation, were not able to detect dependencies at the selected significance level. In contrast, mutual information, which measures more general statistical dependencies in terms of shared information, revealed highly significant dependencies for almost all variable pairs. The weakest statistical relation was found between winds speed and CH${}_4$ emission, but the convection regime was found to play a key role. Clustering was consistent among the three data sets with five typical clusters related to high/low temperature and wind speed, respectively, as well as in some cases to morning and evening hours. Our analysis showed that despite the disparate and often insignificant correlation between environmental variables and CH${}_4$ emission, there is a strong relation between both, which shapes the emission pattern in many aspects much more in addition to differences in the floor type. Although a clear distinction of high and low emission condition clusters based on the selected environmental variables was not possible, trends were clearly visible. Further research with larger data sets is advisable to verify the detected trends and enable prognoses for husbandry systems under different climate conditions. Full article

An integrated method is required for comprehensive assessment of the environmental impacts and economic benefits of rice production systems. Therefore, the objective of this study was to apply different footprinting approaches (carbon footprint (CF), nitrogen footprint (NF), water footprint (WF)) and determine the economic return on organic rice farming (OF) and conventional rice farming (CVF) at the farm scale. Over the 4-year study period (2018–2021), the results showed lower net greenhouse gas (GHG) emissions in OF (3289.1 kg CO₂eq ha⁻¹ year⁻¹) than in CVF (4921.7 kg CO₂eq ha⁻¹ year⁻¹), indicating that the use of OF can mitigate the GHG emissions from soil carbon sequestration. However, there was a higher CF intensity in OF (1.17 kg CO₂eq kg⁻¹ rice yield) than in CVF (0.93 kg CO₂eq kg⁻¹ rice yield) due to the lower yield. The NF intensities of OF and CVF were 0.34 and 11.94 kg Neq kg⁻¹ rice yield, respectively. The total WF of CVF (1470.1 m³ ton⁻¹) was higher than that in OF (1216.3 m³ ton⁻¹). The gray water in CVF was significantly higher than that in OF due to the use of chemical fertilizers, herbicides, and pesticides. Although the rice yield in OF was nearly two times lower than that in CVF, the economic return was higher due to lower production costs and higher rice prices. However, more field studies and long-term monitoring are needed for future research. Full article

The aim of the research was to determine the effect of fertilising with various doses of ash from biomass combustion (D2–D6) compared to control plots and classic NPK (D1) fertiliser on the morphological and mechanical properties of potato tubers (Solanum tuberosum L.). The field experiment was carried out in the years 2019–2021 (south-eastern Poland, 49°59′ N, 21°57′ E) on two types of soil: Gleyic Chernozem (silty loam) and Haplic Luvisol (silt). The values of mechanical parameters, such as the peel and flesh punching force (F_D), deformation (D_R) and energy (E_D) needed to destroy the test sample, were assessed. The biometric features of the tubers were also assessed. It was found that tubers obtained from the experimental fields where D3 and D4 ash fertiliser doses were applied (corresponding to doses of 188 and 282 kg∙ha⁻¹ K) had the highest tuber yields and the highest resistance to mechanical damage under quasi-static loads. Ash from biomass combustion can be an alternative to conventional mineral fertilizers and can be used in the development of mineral fertilization plans for sustainable agriculture, which will help to solve the problem of storage of this waste. Full article

Biochar has the potential for improving soil properties and supporting ecological functions, but it has negative impacts on soil organisms in some cases. This study aimed to assess the effect of biochar application at rates of 0 (B0), 5 Mg ha⁻¹ (B5), 20 Mg⁻¹ (B20), and 40 Mg ha⁻¹ (B40) on soil nematode community under upland and flooded conditions in a short-term microcosm experiment. After biochar application, soil was incubated for 2 to 8 weeks and nematodes were identified for community composition, trophic structures, functional guilds, maturity index and metabolic footprints. The chemical properties of the soils were also analyzed. General linear model revealed that biochar increased soil pH, EC, NO₃⁻-N, available phosphorus, total C, and C/N ratio, particularly in the highest application rate and shifted the composition of nematodes. The greatest abundances of omnivores (Mesodorylaimus, Thornenema), predator (Nygolaimus) and functional guilds of cp5 were observed in B5, resulting in greatest structure footprint and composite footprint, (omnivorous + predator) footprint and total biomass in B5. While abundances of nematodes tended to decrease with the biochar applicate rates, the abundance of Prismatolaimus was the highest in B40. During the 8-week incubation period, the abundances of Achromadora, Alaimus, Aporcelaimellus, Cryptonchus, Mononchus, and Tobrilus remained stable in upland conditions. Under flooded conditions, the abundances of almost all taxa were markedly lower than those under upland conditions irrespective of biochar application, except for Acrobeloides, Alaimus, Aphelenchoides, and Ditylenchus. We highlighted that 5 Mg ha⁻¹ of rice husk biochar can be the optimum in shaping the nematode community. Full article

Weed competitiveness in wheat (Triticum aestivum L.) has previously been shown to be positively associated with shoot biomass. This study evaluated the impact of increased early shoot vigour on the weed competitiveness of Australian wheats. Breeding lines generated for early shoot vigour were top-crossed with two commercial wheat cultivars (Yitpi and Wyalkatchem) and the resulting high vigour lines (HV lines) were assessed for early growth and weed pressure in the field. These lines were directly compared with their parental lines, other commercial cultivars, and the tall heritage cultivar, Federation. Moreover, rye (Secale cereale L.) or triticale (× Triticosecale) was included in each trial as a positive control for vigour. The association between shoot growth and vigour and weed suppression was evaluated over 3 years in the cereal belt of south-eastern Australia during contrasting seasons. The HV lines consistently displayed greater leaf area, ground cover, and canopy light interception in both dry and wet seasons and suppressed weed growth significantly in contrast to commercial cultivars. Light interception at the first tiller stage, and ground cover at the end of tillering were identified as the most important variables for predicting weed suppression. This study demonstrated the enhancement of competitiveness in commercial wheat through the selection for early vigour, and identified traits that best predicted weed suppression. Full article