Nematostella vectensis, an Emerging Model for Deciphering the Molecular and Cellular Mechanisms Underlying Whole-Body Regeneration
Abstract
:1. Introduction
2. Phylogeny, Morphology, Reproduction, and Homeostatic Plasticity of Nematostella vectensis
3. Available Resources, Techniques, and Tools to Study Nematostella vectensis
4. The Morphological, Cellular, and Molecular Basis of Regeneration in Nematostella
4.1. Morphological and Tissular Dynamics
4.2. Cellular Dynamics Underlying Nematostella Regeneration
4.3. Molecular Wound-Healing Response and Patterning during Nematostella Regeneration
4.4. Regeneration-Specific Genes and Gene Modules
5. Conclusions and Future Directions
Funding
Acknowledgments
Conflicts of Interest
References
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Culture and Experimentation | |
Rearing and spawning | [28,31,32] |
Inducing and assessing regeneration | [33,34,35] |
Microinjection and micromanipulation | [36,37,38,39,40,41,42,43,44,45,46,47] |
Genome, Resources, and Protocols for -Omics Analyses | |
Annotated genome—V1.0 | [18] https://mycocosm.jgi.doe.gov/Nemve1/Nemve1.home.html (accessed on 5 October 2021) |
Annotated genome—V2.0 | [48] https://genomes.stowers.org/starletseaanemone (accessed on 5 October 2021) |
Microarrays | [49,50,51,52] |
RNA-seq/transcriptomes | [20,53,54] http://nvertx.kahikai.org (accessed on 5 October 2021) |
scRNA-seq | [55] |
ATAC-seq | [55] |
ChIP-seq | [56] |
Spatio–Temporal Gene Expression Analysis | |
mRNA in situ | [57,58,59,60,61,62,63,64] |
Immunohistochemistry | [36,37,39,40,46,65,66,67] |
Transgenic reporter | [68,69,70,71,72,73] |
Tools to Study Gene Function | |
mRNA over-expression | [40,47,49,51,65] |
Morpholino | [36,39,41,46,47,49,51,63,65] |
Short hairpin RNA | [74,75] |
TALEN/Fok1, CRISPR/Cas9 | [69] |
Heat-shock inducible promoters | [69] |
Pharmaceutical treatments | [21,39,51,71,76,77,78,79,80] |
Available Stable Reporter Lines | |
NvMyHC::mCherry | [68] |
NvElav::mOrange | [81] |
NvElav::Cerulean | [82] |
NvLWamide:mCherry | [73] |
NvEf1a::mOrange-CAAX | [83] |
NvFoxQ2d::mOrange-CAAX | [82] |
NvNcol-3::memOrange2 | [84] |
NvAnthox8::Gfp | [74] |
NvPou4::mCherry | [70] |
NvTBP::mCherry | [85] |
Available Stable KO Lines | |
NvAnthox1a−/− | [74] |
NvAnthox6−/− | [74] |
NvAnthox8a−/− | [74] |
NvFgfrB+/− | [71] |
NvPou4+/− | [70] |
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Röttinger, E. Nematostella vectensis, an Emerging Model for Deciphering the Molecular and Cellular Mechanisms Underlying Whole-Body Regeneration. Cells 2021, 10, 2692. https://doi.org/10.3390/cells10102692
Röttinger E. Nematostella vectensis, an Emerging Model for Deciphering the Molecular and Cellular Mechanisms Underlying Whole-Body Regeneration. Cells. 2021; 10(10):2692. https://doi.org/10.3390/cells10102692
Chicago/Turabian StyleRöttinger, Eric. 2021. "Nematostella vectensis, an Emerging Model for Deciphering the Molecular and Cellular Mechanisms Underlying Whole-Body Regeneration" Cells 10, no. 10: 2692. https://doi.org/10.3390/cells10102692