With-No-Lysine Kinase 1 (WNK1) Augments TRPV4 Function in the Aldosterone-Sensitive Distal Nephron
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents and Animals
2.2. Isolation and Split-Opening of Collecting Ducts
2.3. Cell Culture
2.4. [Ca2+]i Imaging
2.5. Isolation of TRPV4 Membrane Fraction
2.6. Western Blotting
2.7. Over-Expression of TRPV4 in Chinese Hamster Ovary (CHO) Cells
2.8. Data Analysis and Presentation
3. Results
3.1. WNK Blockade Decreases TRPV4 Activity and Expression in the ASDN
3.2. WNK1 Is Critical for Stimulation of TRPV4 by Aldosterone in Cultured mpkCCDc14 Cells
3.3. WNK1 Increases TRPV4 Activity in a Kinase-Dependent Manner
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Tomilin, V.N.; Pyrshev, K.; Khayyat, N.H.; Zaika, O.; Pochynyuk, O. With-No-Lysine Kinase 1 (WNK1) Augments TRPV4 Function in the Aldosterone-Sensitive Distal Nephron. Cells 2021, 10, 1482. https://doi.org/10.3390/cells10061482
Tomilin VN, Pyrshev K, Khayyat NH, Zaika O, Pochynyuk O. With-No-Lysine Kinase 1 (WNK1) Augments TRPV4 Function in the Aldosterone-Sensitive Distal Nephron. Cells. 2021; 10(6):1482. https://doi.org/10.3390/cells10061482
Chicago/Turabian StyleTomilin, Viktor N., Kyrylo Pyrshev, Naghmeh Hassanzadeh Khayyat, Oleg Zaika, and Oleh Pochynyuk. 2021. "With-No-Lysine Kinase 1 (WNK1) Augments TRPV4 Function in the Aldosterone-Sensitive Distal Nephron" Cells 10, no. 6: 1482. https://doi.org/10.3390/cells10061482