Toxoplasma gondii Rhoptry Protein 7 (ROP7) Interacts with NLRP3 and Promotes Inflammasome Hyperactivation in THP-1-Derived Macrophages
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Lines and Parasite Strains
2.2. Reagents and Antibodies
2.3. Plasmid Construction
2.4. Co-Immunoprecipitation Assays and Liquid Chromatography/Mass Spectrometry (LC/MS) Screening
2.5. Western Blotting
2.6. Enzyme-Linked Immunosorbent Assay (ELISA)
2.7. NLRP3 Inflammasome Reconstitution in 293T Cells
2.8. Confocal Microscope Analysis of Protein Co-Localization
2.9. Generation of Human THP-1 Cell Lines Expressing ROP7 Stably
2.10. LDH Assay
2.11. qPCR
2.12. NF-κB Dual-Luciferase Reporter Gene Assay
2.13. T. gondii ME49 ROP7 Knockout
2.14. Plaque Assays
2.15. THP-1 Infection with T. gondii
2.16. Statistical Analysis
3. Results
3.1. T. gondii Secretory Protein ROP7 Is Associated with NLRP3 and Promotes the Maturation of IL-1β in 293T Cells
3.2. NLRP3 and ROP7 Interact through the NACHT Domain and ROP7AA35-255
3.3. ROP7 Expression in THP-1 Induces NLRP3 Inflammasome Hyperactivation
3.4. ROP7 in THP-1 Triggers the Priming Signal but Not Derived from ROP7 Itself to NF-κB Pathway Directly
3.5. The IL-1β/NF-κB/NLRP3-Positive Feedback Loop Participates in ROP7-Induced Inflammasome Activation
3.6. ROP7 and the IL-1β/NF-κB/NLRP3 Loop Are Involved in Parasite-Induced Inflammasome Response in Macrophages
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhu, L.; Qi, W.; Yang, G.; Yang, Y.; Wang, Y.; Zheng, L.; Fu, Y.; Cheng, X. Toxoplasma gondii Rhoptry Protein 7 (ROP7) Interacts with NLRP3 and Promotes Inflammasome Hyperactivation in THP-1-Derived Macrophages. Cells 2022, 11, 1630. https://doi.org/10.3390/cells11101630
Zhu L, Qi W, Yang G, Yang Y, Wang Y, Zheng L, Fu Y, Cheng X. Toxoplasma gondii Rhoptry Protein 7 (ROP7) Interacts with NLRP3 and Promotes Inflammasome Hyperactivation in THP-1-Derived Macrophages. Cells. 2022; 11(10):1630. https://doi.org/10.3390/cells11101630
Chicago/Turabian StyleZhu, Lijun, Wanjun Qi, Guang Yang, Yurong Yang, Yuwen Wang, Lulu Zheng, Yongfeng Fu, and Xunjia Cheng. 2022. "Toxoplasma gondii Rhoptry Protein 7 (ROP7) Interacts with NLRP3 and Promotes Inflammasome Hyperactivation in THP-1-Derived Macrophages" Cells 11, no. 10: 1630. https://doi.org/10.3390/cells11101630