Genotype-by-Environment Interaction Analysis of Metabolites in Pearl Millet Genotypes with High Concentrations of Slowly Digestible and Resistant Starch in Their Grains
, Prakash I. Gangashetty 2, Manfred Beckmann 1, Luis A. J. Mur 1 and Rattan S. Yadav 1,*Round 1
Reviewer 1 Report
The study was focused around an important area of metabolite diversity in pearl millet, an important crop in the developing world. The study is conducted methodically and the results are explained in a lucid manner. The assessment of eleven pearl millet hybrids which were earlier identified for possessing high concentrations of SDS and RS in their grains. More importantly, the stable performance of these hybrids was assessed across five different environmental conditions. The useful outcome of the study is that hybrids were chosen for resistant starch and slowly digestible starch which are important to have low GI in pearl millet. The contribution of this study is significant in view of its practical applications.
Certain syntax errors have been marked in the text. Sentences which are incomplete are marked in the text. Manuscript needs to be edited for the language.
This manuscript is accepted for publication after incorporating all the suggested corrections. Please check the file attached for corrections to be incorporated.
Comments for author File: 
Comments.pdf
Author Response
Comment 1: Page 1, line 32-33; These findings are going to be,,,, will helpful
Reply: Page 1, line 32-33; will,,,deleted in the sentence.
Comment 2: Page 2, line 87-88 “performed from frozen milled grain samples of eleven accession of pearl millet”
Reply: Page 2, line 87-88, replaced with “performed from frozen milled grain samples of eleven accessions of pearl millet”
Comment 3: Page 6, line 217-218, significant (G), (E) and (GEI) differences
Reply: Page 6, line 217-218, as per suggestions the sentences modified in the MS “significant (G, genotype), (E, environment), and (GEI, genotype and environment interaction) differences”
Comment 4: Page 6, line 237, GEI (genotype and environment interaction)
Reply: Page 6, line 237, GEI expansion has been deleted “(genotype and environment interaction)”
Comment 5: Page 6, line 244-245, “showed the maximum variance with the first PC (which explained 91.2% toward the total GEI)”
Reply: Page 6, line 244-245, the sentences replaced with “showed the maximum variance with the first PC (which explained 91.2% towards the total GEI)”
Comment 6: Page 6, line 250, toward
Reply: Page 6, line 250, toward replaced with towards
Comment 7: Page 6, line 267, environment
Reply: Page 6, line 267, “environment” replaced with “environmental”
Comment 8: Page 7, line 279, environment
Reply: Page 7, line 279, “environment” replaced with “environments”
Comment 9: Page 8, line 288, were
Reply: Page 8, line 288, “were” replaced with “was”
Comment 10: Page 8, line 295, However, contrastingly unfavourable environmental effects for starch metabolism-related (m/z 539.13831) metabolite (Figure 3a).
Reply: Page 8, line 295, Sentence was modified in the MS “However, contrastingly unfavourable environmental effects were observed for starch metabolism-related (m/z 539.13831) metabolite (Figure 3a).”
Comment 11: Page 9, line 339, “such as”
Reply: Page 9, line 339, as per suggestions “such as” deleted in the MS
Comment 12: Page 9, line 343, “WAASB”
Reply: Page 9, line 339, as per suggestions “WAASB” replaced with “WAASB method” in the MS
Comment 13: Page 10, line 360, Millets are particularly pearl millets important due to their critical role in human nutrition.
Reply: Page 10, line 360, the sentence has been modified “Millets particularly pearl millets are important due to their critical role in human nutrition.
Comment 14: Page 10, line 374, “role”
Reply: Page 10, line 374, “role” replaced with “roles”
Comment 15: Page 11, line 417, “BLUP-based”
Reply: Page 11, line 417, BLUP-based” replaced with “BLUP-based models”
Comment 16: Page 12, line 439, “this”
Reply: Page 12, line 439, “this” replaced with “the”
Comment 13: English correction
Reply: As suggested by reviewer - The MS has been read and corrected by native English speaker.
Reviewer 2 Report
Manuscript is well composed however minor sentence formation issues are there that need to be checked.
Comments for author File: Comments.pdf
Author Response
Comment 1: Page 12, line 439, rephrasing of sentences
Reply: The sentences has been modified in the MS
Comment 1: English correction
Reply: As suggested by reviewer - The MS has been read and corrected by native English speaker.
