Large-Scale, Wavelet-Based Analysis of Lysosomal Trajectories and Co-Movements of Lysosomes with Nanoparticle Cargos
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture, Nanoparticle Treatments, and Confocal Microscopy
2.2. Image Processing and Tracking
2.3. Movement Analysis
2.3.1. Continuous Wavelet Transform
2.3.2. Active Transport Detection
2.3.3. Co-Movement Detection
2.4. Statistical Data Analysis
3. Results
3.1. Lysosomal Movements Are Characterized by a Heavy-Tailed, Lognormal Distribution of Run/Flight Lengths
3.2. Tissue Origin and Cancer-Specific Differences in Lysosomal Dynamics
3.3. Wavelet-Based Approach for Detection of Lysosome-Nanoparticle Co-Movement
3.4. Mixed-Charge Gold Nanoparticles Selectively Disrupt Lysosomal Transport in Cancer Cells
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Appendix A
Appendix B
Distribution Name | Probability Density Function p(x) |
---|---|
Power law | |
Truncated power law | |
Log-normal | |
Stretched exponential | |
Exponential |
Cell Type/Treatment | Lysosomal Diameter [μm] | Lysosomes, l | Cells, n |
---|---|---|---|
MEF | 0.65 ± 0.23 | 525 | 7 |
MEF + 80:20 NPs | 0.71 ± 0.25 * | 759 | 6 |
HT-1080 | 0.67 ± 0.24 | 707 | 6 |
HT-1080 + 80:20 NPs | 0.99 ± 0.34 * | 539 | 11 |
MCF-10A | 0.54 ± 0.20 | 316 | 10 |
MCF-10A + 80:20 NPs | 0.57 ± 0.22 ns | 340 | 12 |
MDA-MB-231 | 0.67 ± 0.30 | 418 | 16 |
MDA-MB-231 + 80:20 NPs | 1.00 ± 0.49 * | 235 | 10 |
MCF-7 | 0.80 ± 0.35 | 250 | 11 |
MCF-7 + 80:20 NPs | 1.42 ± 0.92 * | 239 | 11 |
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Cell Type | MSD (a) | D (μm2/s) | Fit Parameters | Akaike Weights | ||||
---|---|---|---|---|---|---|---|---|
LN | P | TP | SE | E | ||||
MCF-10A | 1.29 ± 0.05 | 0.022 ± 0.004 | Runs: μ = −0.193; σ = 0.804 Flights: λ = 0.840; β = 1.037 | 1 0 | 0 <0.01 | <0.01 <0.01 | <0.01 0.83 | <0.01 0.17 |
MDA-MB-231 | 1.31 ± 0.05 | 0.029 ± 0.006 | Runs: μ = −0.147; σ = 0.833 Flights: μ = 0.028; σ = 0.838 | 0.99 1 | <0.01 <0.01 | <0.01 <0.01 | <0.01 <0.01 | <0.01 <0.01 |
MCF-7 | 1.35 ± 0.04 | 0.029 ± 0.010 | Runs: μ = −0.013; σ = 0.834 Flights: μ = 0.151; σ = 0.838 | 1 1 | 0 0 | 0 <0.01 | <0.01 <0.01 | <0.01 <0.01 |
MEF | 1.36 ± 0.09 | 0.018 ± 0.003 | Runs: μ = −0.175; σ = 0.856 Flights: μ = −0.155; σ = 0.929 | 1 0.99 | 0 0 | <0.01 <0.01 | <0.01 <0.01 | <0.01 <0.01 |
HT-1080 | 1.34 ± 0.06 | 0.015 ± 0.003 | Runs: μ = −0.183; σ = 0.802 Flights: μ = −0.019; σ = 0.829 | 1 1 | 0 0 | 0 0 | <0.01 <0.01 | <0.01 <0.01 |
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Polev, K.; Kolygina, D.V.; Kandere-Grzybowska, K.; Grzybowski, B.A. Large-Scale, Wavelet-Based Analysis of Lysosomal Trajectories and Co-Movements of Lysosomes with Nanoparticle Cargos. Cells 2022, 11, 270. https://doi.org/10.3390/cells11020270
Polev K, Kolygina DV, Kandere-Grzybowska K, Grzybowski BA. Large-Scale, Wavelet-Based Analysis of Lysosomal Trajectories and Co-Movements of Lysosomes with Nanoparticle Cargos. Cells. 2022; 11(2):270. https://doi.org/10.3390/cells11020270
Chicago/Turabian StylePolev, Konstantin, Diana V. Kolygina, Kristiana Kandere-Grzybowska, and Bartosz A. Grzybowski. 2022. "Large-Scale, Wavelet-Based Analysis of Lysosomal Trajectories and Co-Movements of Lysosomes with Nanoparticle Cargos" Cells 11, no. 2: 270. https://doi.org/10.3390/cells11020270