RNA N6-Methyladenosine (m6A) Methyltransferase-like 3 Facilitates Tumorigenesis and Cisplatin Resistance of Arecoline-Exposed Oral Carcinoma
Abstract
:1. Introduction
2. Materials and Methods
2.1. Human Sample Collection
2.2. Cell Culture, Cell Transfection, and Lentiviral Transduction
2.3. Immunohistochemistry (IHC) Analysis
2.4. Luciferase Reporter Assay
2.5. RT-qPCR
2.6. Chromatin Immunoprecipitation (ChIP)–qPCR Assay
2.7. Western Blotting
2.8. Cell Proliferation Assay and Cell Migration Assay
2.9. ELISA
2.10. Mouse Tumorigenesis and Treatment
2.11. Statistical Analyses
3. Results
3.1. MTTL3 Is Elevated in Arecoline-Exposed OSCC Cells and Tissues
3.2. HIF-1α Regulates METTL3 Expression
3.3. HIF-1α is Enhanced in Arecoline-Exposed OSCC Cells and Tissues
3.4. METTL3-MYC- HIF-1α Forms a Positive Autoregulation Loop
3.5. Knocking down METTL3 Sensitized Arecoline-Transformed OSCC Cells to Cisplatin-Induced Cytotoxicity
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Relative METTL3 mRNA Levels | p-Value | |
---|---|---|
Gender | ||
Female | 1.83 | 0.061 |
Male | 2.06 | |
Age | ||
<65 | 2.01 | 0.83 |
≥65 | 2.05 | |
Areca nut chewing habit | ||
Yes | 1.75 | 0.041 |
No | 2.18 | |
Tumor Stage | ||
1 and 2 | 1.89 | 0.055 |
3 and 4 | 2.01 | |
Nodal Stage | ||
0 | 1.83 | 0.073 |
1 | 1.95 | |
2 | 2.02 | |
Recurrence | ||
No | 1.24 | 0.034 |
Local | 1.66 | |
Distant | 2.03 |
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Wang, C.; Kadigamuwa, C.; Wu, S.; Gao, Y.; Chen, W.; Gu, Y.; Wang, S.; Li, X. RNA N6-Methyladenosine (m6A) Methyltransferase-like 3 Facilitates Tumorigenesis and Cisplatin Resistance of Arecoline-Exposed Oral Carcinoma. Cells 2022, 11, 3605. https://doi.org/10.3390/cells11223605
Wang C, Kadigamuwa C, Wu S, Gao Y, Chen W, Gu Y, Wang S, Li X. RNA N6-Methyladenosine (m6A) Methyltransferase-like 3 Facilitates Tumorigenesis and Cisplatin Resistance of Arecoline-Exposed Oral Carcinoma. Cells. 2022; 11(22):3605. https://doi.org/10.3390/cells11223605
Chicago/Turabian StyleWang, Chuang, Chamila Kadigamuwa, Songlv Wu, Yijun Gao, Wuya Chen, Yangcong Gu, Shengli Wang, and Xia Li. 2022. "RNA N6-Methyladenosine (m6A) Methyltransferase-like 3 Facilitates Tumorigenesis and Cisplatin Resistance of Arecoline-Exposed Oral Carcinoma" Cells 11, no. 22: 3605. https://doi.org/10.3390/cells11223605