The Impact of CC16 on Pulmonary Epithelial-Driven Host Responses during Mycoplasma pneumoniae Infection in Mouse Tracheal Epithelial Cells
Abstract
:1. Introduction
2. Results
2.1. WT MTECs Have Decreased Apical Proteins Secretions during Mp Infection
2.2. WT MTECs Have Decreased Secretion of Antioxidant Proteins during Mp Infection
2.3. CC16−/− MTECs Have Increased Apical Protein Secretions during Mp Infection
2.4. CC16−/− MTECs Have Increased Secretion of Airway Remodeling and Bacterial Invasion Proteins during Mp Infection
2.5. CC16 Mediates Pulmonary Epithelial Cell Apical Protein Secretion during Mp Infection
2.6. CC16 Deficiency Increases Pulmonary Epithelial-Driven Airway Remodeling during Mp Infection
2.7. Validation of Apically Secreted Proteins in MTECs and Mouse Lung Tissue by Western Blot
3. Discussion
4. Conclusions
5. Methods
5.1. WT and CC16−/− Mice
5.2. Mouse Tracheal Epithelial Cell (MTEC) Isolation and Culturing
5.3. Mp Infection for WT and CC16−/− MTECs and Mice
5.4. Collection of Apically Secreted Proteins from MTECs
5.5. In-Solution Tryptic Digestion
5.6. Mass Spectrometry and Spectrum Count Data Processing
5.7. Label-Free Quantitative Proteomics
5.8. Determination of GRB2 and LAMB3 Expression by Western Blotting
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Protein Name | Protein Abbreviation | Gene Abbreviation | SNP IDs in Lung Tissue a | Effective Allele | p-Value | Predicted Expression Change Due to SNP within Lung Tissue b |
---|---|---|---|---|---|---|
Heparin Sulfate Proteoglycan 2 | PGBM | HSPG2 | rs2229475 | T | 5.58 × 10−14 | Increase |
rs115963344 | T | 4.84 × 10−13 | Increase | |||
rs12724454 | A | 4.84 × 10−13 | Increase | |||
rs12725566 | A | 4.84 × 10−13 | Increase | |||
rs12737091 | T | 4.84 × 10−13 | Increase | |||
rs12741617 | A | 4.84 × 10−13 | Increase | |||
rs12742444 | T | 4.84 × 10−13 | Increase | |||
Adapter Molecule CRK | CRK | CRK | rs16946807 | A | 3.74 × 10−8 | Decrease |
rs34490967 | C | 5.86 × 10−8 | Decrease | |||
rs7208768 | A | 8.46 × 10−8 | Decrease | |||
rs11652967 | G | 9.72 × 10−8 | Decrease | |||
rs35316912 | C | 1.16 × 10−7 | Decrease | |||
Ras GTPase-Activating-Like Protein | IQGA2 | IQGAP2 | rs112872685 | A | 1.84 × 10−7 | Decrease |
rs875541 | A | 9.95 × 10−6 | Decrease | |||
rs10075621 | T | 1.40 × 10−5 | Decrease | |||
rs4296785 | T | 1.42 × 10−5 | Decrease | |||
rs4501323 | C | 1.42 × 10−5 | Decrease | |||
rs4501324 | C | 1.42 × 10−5 | Decrease | |||
Syndecan-4 | SDC4 | SDC4 | rs2267867 | A | 3.10 × 10−8 | Increase |
Laminin Subunit Beta 3 | LAMB3 | LAMB3 | rs1534962 | A | 5.22 × 10−14 | Decrease |
rs9429823 | G | 1.08 × 10−12 | Increase | |||
rs2298928 | T | 2.05 × 10−12 | Increase | |||
rs2298926 | A | 3.74 × 10−12 | Increase | |||
rs1534962 | A | 5.63 × 10−12 | Decrease | |||
rs9429823 | G | 5.12 × 10−9 | Increase | |||
Plexin-B2 | PLXB2 | PLXNB2 | rs74933135 | A | 3.36 × 10−8 | Increase |
rs117289563 | T | 1.05 × 10−7 | Increase | |||
rs118067161 | T | 1.05 × 10−7 | Increase | |||
rs142227764 | T | 1.05 × 10−7 | Increase | |||
rs78854007 | A | 1.05 × 10−7 | Increase | |||
rs146310944 | T | 1.05 × 10−7 | Increase | |||
rs183166435 | C | 1.05 × 10−7 | Increase | |||
rs117199604 | A | 2.33 × 10−7 | Increase | |||
Growth Factor Receptor-Bound Protein 2 | GRB2 | GRB2 | rs12451296 | A | 3.33 × 10−5 | Increase |
rs11649784 | T | 3.54 × 10−5 | Increase | |||
rs12604003 | A | 3.54 × 10−5 | Increase | |||
rs4789187 | G | 3.54 × 10−5 | Increase | |||
rs9895739 | T | 3.66 × 10−5 | Increase | |||
rs9909443 | T | 4.02 × 10−5 | Decrease | |||
rs9901434 | G | 4.18 × 10−5 | Increase | |||
Dynactin Subunit 1 | DCTN1 | DCTN1 | rs34215278 | T | 5.19 × 10−16 | Decrease |
rs11555696 | A | 6.86 × 10−16 | Decrease | |||
rs71418733 | T | 6.86 × 10−16 | Decrease | |||
rs71418738 | G | 1.56 × 10−15 | Decrease | |||
rs13021268 | T | 9.16 × 10−15 | Decrease | |||
rs34988074 | C | 1.12 × 10−14 | Decrease | |||
rs34522471 | G | 1.10 × 10−11 | Decrease | |||
rs35241844 | C | 1.00 × 10−9 | Decrease | |||
Protein BRICK1 | BRK1 | BRK1 | rs67342818 | A | 8.10 × 10−9 | Decrease |
rs111284032 | T | 8.10 × 10−9 | Decrease | |||
rs112180322 | T | 8.10 × 10−9 | Decrease | |||
rs113378528 | A | 8.10 × 10−9 | Decrease | |||
rs3774208 | G | 8.10 × 10−9 | Decrease | |||
rs41464050 | G | 8.10 × 10−9 | Decrease | |||
rs58862481 | A | 8.10 × 10−9 | Decrease | |||
Clathrin Light Chain B | CLCB | CLTB | rs13181024 | T | 2.97 × 10−32 | Increase |
rs13168842 | T | 2.97 × 10−32 | Increase | |||
rs13154366 | A | 2.19 × 10−25 | Increase | |||
rs13175787 | G | 2.19 × 10−25 | Increase | |||
rs13168952 | A | 5.90 × 10−25 | Increase | |||
rs13180938 | A | 2.47 × 10−23 | Increase | |||
rs13181024 | T | 1.94 × 10−22 | Increase | |||
rs13168842 | T | 1.94 × 10−22 | Increase | |||
Integrin-Linked Protein Kinase | ILK | ILK | rs11826498 | C | 4.89 × 10−15 | Increase |
rs11602107 | A | 1.17 × 10−14 | Increase | |||
rs11605114 | A | 1.17 × 10−14 | Increase | |||
rs2255405 | A | 1.56 × 10−14 | Increase | |||
rs12451 | T | 2.62 × 10−14 | Increase | |||
rs3741271 | C | 4.63 × 10−14 | Increase | |||
rs2255538 | A | 9.88 × 10−14 | Increase | |||
rs2292195 | T | 1.80 × 10−13 | Increase |
Gene | SNP ID | Risk Allele | Expression Change | Effect of SNP on Phenotype | p-Value |
---|---|---|---|---|---|
GRB2 | rs9901434 | G | ↑ | ↓ FEV1 | 6.53 × 10−6 |
↑ asthma risk | 2.33 × 10−2 | ||||
rs9895739 | T | ↑ | ↓ FEV1 | 4.26 × 10−6 | |
↑ asthma risk | 3.10 × 10−2 | ||||
rs11649784 | T | ↑ | ↑ asthma risk | 2.22 × 10−2 | |
rs12604003 | A | ↑ | ↑ asthma risk | 2.13 × 10−2 | |
rs4789187 | G | ↑ | ↑ asthma risk | 2.22 × 10−2 |
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Iannuzo, N.; Dy, A.B.C.; Guerra, S.; Langlais, P.R.; Ledford, J.G. The Impact of CC16 on Pulmonary Epithelial-Driven Host Responses during Mycoplasma pneumoniae Infection in Mouse Tracheal Epithelial Cells. Cells 2023, 12, 1984. https://doi.org/10.3390/cells12151984
Iannuzo N, Dy ABC, Guerra S, Langlais PR, Ledford JG. The Impact of CC16 on Pulmonary Epithelial-Driven Host Responses during Mycoplasma pneumoniae Infection in Mouse Tracheal Epithelial Cells. Cells. 2023; 12(15):1984. https://doi.org/10.3390/cells12151984
Chicago/Turabian StyleIannuzo, Natalie, Alane Blythe C. Dy, Stefano Guerra, Paul R. Langlais, and Julie G. Ledford. 2023. "The Impact of CC16 on Pulmonary Epithelial-Driven Host Responses during Mycoplasma pneumoniae Infection in Mouse Tracheal Epithelial Cells" Cells 12, no. 15: 1984. https://doi.org/10.3390/cells12151984