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Article

Improved Method for Dental Pulp Stem Cell Preservation and Its Underlying Cell Biological Mechanism

by
Mai Takeshita-Umehara
,
Reiko Tokuyama-Toda
*,
Yusuke Takebe
,
Chika Terada-Ito
,
Susumu Tadokoro
,
Akemi Inoue
,
Kohei Ijichi
,
Toshio Yudo
and
Kazuhito Satomura
Department of Oral Medicine and Stomatology, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Kanagawa, Japan
*
Author to whom correspondence should be addressed.
Cells 2023, 12(17), 2138; https://doi.org/10.3390/cells12172138
Submission received: 22 June 2023 / Revised: 22 August 2023 / Accepted: 23 August 2023 / Published: 24 August 2023
(This article belongs to the Section Cell Methods)
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Abstract

Dental pulp stem cells (DPSCs) are considered a valuable cell source for regenerative medicine because of their high proliferative potential, multipotency, and availability. We established a new cryopreservation method (NCM) for collecting DPSCs, in which the tissue itself is cryopreserved and DPSCs are collected after thawing. We improved the NCM and developed a new method for collecting and preserving DPSCs more efficiently. Dental pulp tissue was collected from an extracted tooth, divided into two pieces, sandwiched from above and below using cell culture inserts, and cultured. As a result, the cells in the pulp tissue migrated vertically over time and localized near the upper and lower membranes over 2–3 days. With regard to the underlying molecular mechanism, SDF1 was predominantly involved in cell migration. This improved method is valuable and enables the more efficient collection and reliable preservation of DPSCs. It has the potential to procure a large number of DPSCs stably.
Keywords: dental pulp stem cell; regenerative medicine; preservation method dental pulp stem cell; regenerative medicine; preservation method

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MDPI and ACS Style

Takeshita-Umehara, M.; Tokuyama-Toda, R.; Takebe, Y.; Terada-Ito, C.; Tadokoro, S.; Inoue, A.; Ijichi, K.; Yudo, T.; Satomura, K. Improved Method for Dental Pulp Stem Cell Preservation and Its Underlying Cell Biological Mechanism. Cells 2023, 12, 2138. https://doi.org/10.3390/cells12172138

AMA Style

Takeshita-Umehara M, Tokuyama-Toda R, Takebe Y, Terada-Ito C, Tadokoro S, Inoue A, Ijichi K, Yudo T, Satomura K. Improved Method for Dental Pulp Stem Cell Preservation and Its Underlying Cell Biological Mechanism. Cells. 2023; 12(17):2138. https://doi.org/10.3390/cells12172138

Chicago/Turabian Style

Takeshita-Umehara, Mai, Reiko Tokuyama-Toda, Yusuke Takebe, Chika Terada-Ito, Susumu Tadokoro, Akemi Inoue, Kohei Ijichi, Toshio Yudo, and Kazuhito Satomura. 2023. "Improved Method for Dental Pulp Stem Cell Preservation and Its Underlying Cell Biological Mechanism" Cells 12, no. 17: 2138. https://doi.org/10.3390/cells12172138

APA Style

Takeshita-Umehara, M., Tokuyama-Toda, R., Takebe, Y., Terada-Ito, C., Tadokoro, S., Inoue, A., Ijichi, K., Yudo, T., & Satomura, K. (2023). Improved Method for Dental Pulp Stem Cell Preservation and Its Underlying Cell Biological Mechanism. Cells, 12(17), 2138. https://doi.org/10.3390/cells12172138

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