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Article
Peer-Review Record

Stability and Requirement for Thiamin in a Cell Culture Feed Used to Produce New Biological Entities

by Alisa Schnellbächer and Aline Zimmer *
Reviewer 1: Anonymous
Reviewer 2:
Submission received: 12 December 2022 / Revised: 1 January 2023 / Accepted: 11 January 2023 / Published: 16 January 2023
(This article belongs to the Special Issue The Molecular Application of Mass Spectrometry in Health and Disease)

Round 1

Reviewer 1 Report

This paper presents data on the degradation of thiamin under certain conditions and metabolites resulting therefrom. It also demonstrates that supplementary thiamin may not be necessary in culture media. 

The paper is very well presented and the conclusions are appropriate based on the experimental data obtained.  The study is detailed and extensive and warrants publication in Cells.

Author Response

Dear Reviewer,

We would like to thank you for taking the time and effort necessary to review the manuscript. We sincerely appreciate your valuable feedback.

Kind regards,

Aline Zimmer

Reviewer 2 Report

1. Fig. 5 has bad quality. Replace it

2. Please provide the choice of chromatographic conditions.

3. Clearly describe the practical significance of the work

4. Rewrite abstact

Author Response

Please see the attachment

Author Response File: Author Response.pdf

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