A Three-Dimensional Engineered Cardiac In Vitro Model: Controlled Alignment of Cardiomyocytes in 3D Microphysiological Systems
Abstract
:1. Introduction
2. Materials and Methods
2.1. Microfabricated Grooves for Cell Alignment
2.2. Hydrogel Preparation
2.3. Cell Culture
2.4. Cell Seeding on PDMS (2D) and in 3D Hydrogel
2.5. Staining and 3D Imaging
2.6. Cell Orientation
2.7. Beating Characteristics
2.8. Statistical Analysis
3. Results
3.1. 3D and 2D Cultures Show Preferential Alignment and Present Elongated Aspect Regardless of Groove Dimension
3.2. The Cell Alignment Propagates in the Grooves Thank to the Contact Guidance
3.3. 3D Patterned Hydrogel Aids a Reliable Measurement of Beating
3.4. Successful Detachability and Stability of the Hydrogel from the PDMS Substrate
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Navaee, F.; Khornian, N.; Longet, D.; Heub, S.; Boder-Pasche, S.; Weder, G.; Kleger, A.; Renaud, P.; Braschler, T. A Three-Dimensional Engineered Cardiac In Vitro Model: Controlled Alignment of Cardiomyocytes in 3D Microphysiological Systems. Cells 2023, 12, 576. https://doi.org/10.3390/cells12040576
Navaee F, Khornian N, Longet D, Heub S, Boder-Pasche S, Weder G, Kleger A, Renaud P, Braschler T. A Three-Dimensional Engineered Cardiac In Vitro Model: Controlled Alignment of Cardiomyocytes in 3D Microphysiological Systems. Cells. 2023; 12(4):576. https://doi.org/10.3390/cells12040576
Chicago/Turabian StyleNavaee, Fatemeh, Niloofar Khornian, David Longet, Sarah Heub, Stephanie Boder-Pasche, Gilles Weder, Alexander Kleger, Philippe Renaud, and Thomas Braschler. 2023. "A Three-Dimensional Engineered Cardiac In Vitro Model: Controlled Alignment of Cardiomyocytes in 3D Microphysiological Systems" Cells 12, no. 4: 576. https://doi.org/10.3390/cells12040576