Identification and Characterization of Multiple Paneth Cell Types in the Mouse Small Intestine
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mice
2.2. PC Sort
2.3. RNA Isolation on Bulk PCs
2.4. PC Bulk RNA-Seq
2.5. PC scRNA-Seq
2.6. Bulk and scRNA-Seq Comparison
2.7. Visualizations
2.8. Tissue Preparation Molecular Cartography
2.9. Molecular Cartography Probe Design
2.10. Molecular CartographyTM
2.11. Defa21 ELISA and Lysozyme Activity Assay
3. Results
3.1. Single-Cell Analysis Unveils Diverse PC Subpopulations
3.2. Regional Variations in PC Transcriptomes along the Small Intestine
3.3. Integrative Analysis of Bulk and Single-Cell Data Links Specific PC Clusters to Regions in the Small Intestine
3.4. Confirmation of PC Clusters via Spatial Transcriptomics
3.4.1. Spatial Transcriptomic Probe Design to Validate PC Clusters
3.4.2. Simple Co-Location Analysis Confirms the Existence of Tuft Cell-like PCs and GC-like PCs
3.4.3. Cell-Based Analysis Identifies Region-Dependent Paneth Cell Identities along the Small Intestine, but Not within the Crypts Themselves
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Timmermans, S.; Wallaeys, C.; Garcia-Gonzalez, N.; Pollaris, L.; Saeys, Y.; Libert, C. Identification and Characterization of Multiple Paneth Cell Types in the Mouse Small Intestine. Cells 2024, 13, 1435. https://doi.org/10.3390/cells13171435
Timmermans S, Wallaeys C, Garcia-Gonzalez N, Pollaris L, Saeys Y, Libert C. Identification and Characterization of Multiple Paneth Cell Types in the Mouse Small Intestine. Cells. 2024; 13(17):1435. https://doi.org/10.3390/cells13171435
Chicago/Turabian StyleTimmermans, Steven, Charlotte Wallaeys, Natalia Garcia-Gonzalez, Lotte Pollaris, Yvan Saeys, and Claude Libert. 2024. "Identification and Characterization of Multiple Paneth Cell Types in the Mouse Small Intestine" Cells 13, no. 17: 1435. https://doi.org/10.3390/cells13171435