Curcumin Inhibits TORC1 and Prolongs the Lifespan of Cells with Mitochondrial Dysfunction
Abstract
:1. Introduction
2. Results
2.1. Curcumin Extends Lifespan of Postmitotic Cells during Chronological Aging
2.2. Curcumin Increases Postmitotic Cellular Lifespan of Mitochondrial Dysfunctional Cells during Chronological Aging in Yeast
2.3. Curcumin Inhibits TORC1 and Prevents Accelerated Aging in Postmitotic Cells with Mitochondrial Dysfunction
2.4. Curcumin Increases Mitochondrial Function and Oxidative Stress
3. Discussion
4. Methods
4.1. Yeast Strains and Culture Conditions
4.2. Chemical Treatments of Cell Cultures
4.3. Analysis of Postmitotic Cellular Lifespan during Chronological Aging in Yeast
4.4. Measurement of ATP Level
4.5. RNA Extraction and qRT-PCR
4.6. Oxidative Stress Growth Sensitivity Assay
4.7. Analysis of TORC1 Activity
4.8. Data Quantification and Statistical Interpretation
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Naaz, A.; Zhang, Y.; Faidzinn, N.A.; Yogasundaram, S.; Dorajoo, R.; Alfatah, M. Curcumin Inhibits TORC1 and Prolongs the Lifespan of Cells with Mitochondrial Dysfunction. Cells 2024, 13, 1470. https://doi.org/10.3390/cells13171470
Naaz A, Zhang Y, Faidzinn NA, Yogasundaram S, Dorajoo R, Alfatah M. Curcumin Inhibits TORC1 and Prolongs the Lifespan of Cells with Mitochondrial Dysfunction. Cells. 2024; 13(17):1470. https://doi.org/10.3390/cells13171470
Chicago/Turabian StyleNaaz, Arshia, Yizhong Zhang, Nashrul Afiq Faidzinn, Sonia Yogasundaram, Rajkumar Dorajoo, and Mohammad Alfatah. 2024. "Curcumin Inhibits TORC1 and Prolongs the Lifespan of Cells with Mitochondrial Dysfunction" Cells 13, no. 17: 1470. https://doi.org/10.3390/cells13171470