Importance of Transcript Variants in Transcriptome Analyses
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Model
2.2. RNA Sequencing Data
2.3. RNA Sequencing Analysis
2.4. Analysis of the Transcript Variants
2.5. Statistical Analyses
3. Results
3.1. Lineage-Specific Expression of Transcription Factors
3.2. Differential Expression of the Transcription Factor Genes and Transcript Variants
3.3. Discrepancy between Gene Expression and Transcript Variants
3.4. Increased Discrepancy among the Low-Abundance Transcript Variants
3.5. The Basis of Discrepancy between Gene Expression and Transcript Variant Analyses
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Vo, K.; Sharma, Y.; Paul, A.; Mohamadi, R.; Mohamadi, A.; Fields, P.E.; Rumi, M.A.K. Importance of Transcript Variants in Transcriptome Analyses. Cells 2024, 13, 1502. https://doi.org/10.3390/cells13171502
Vo K, Sharma Y, Paul A, Mohamadi R, Mohamadi A, Fields PE, Rumi MAK. Importance of Transcript Variants in Transcriptome Analyses. Cells. 2024; 13(17):1502. https://doi.org/10.3390/cells13171502
Chicago/Turabian StyleVo, Kevin, Yashica Sharma, Anohita Paul, Ryan Mohamadi, Amelia Mohamadi, Patrick E. Fields, and M. A. Karim Rumi. 2024. "Importance of Transcript Variants in Transcriptome Analyses" Cells 13, no. 17: 1502. https://doi.org/10.3390/cells13171502