The Interactions of T Cells with Myeloid-Derived Suppressor Cells in Peripheral Blood Stem Cell Grafts
Abstract
:1. Introduction
2. Methods
2.1. Mobilization and Collection of PBSCs
2.2. Graft Analysis
2.3. T Cell Proliferation and Cytokine Secretion from Graft Cells
2.4. Effects of MDSC Subsets on the Proliferation and Expansion of T Cells
2.5. Statistical Analysis
3. Results
3.1. Variation between Individual Grafts and between Different Graft Sources
3.2. Characterization of T Cells in the Grafts
3.3. M-MDSCs Expressed Multiple Ligands of Inhibitory Receptors at Variable Levels and Had Immune Regulatory Function
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Healthy Donor for Allogeneic Transplant (N = 68) | Patient Donor for Autologous Transplant (N = 94) | |
---|---|---|
Age, years, median | 45 (15–67) | Lymphoma: 48 (18–70) Multiple myeloma: 59 (34–71) |
Sex | 44 Male/24 Female | Lymphoma: 12 Male/22 Female Multiple myeloma: 32 Male/29 Female |
Disease | N/A | Lymphoma: HL (11), NHL (23) |
Multiple myeloma: 60 | ||
Mobilization | G-CSF 10 mcg/kg/d × 5 days | Chemotherapy (disease-specific) and G-CSF 10 mcg/kg/d × 5 days |
Days of collection | 1 | 1 |
Total nucleated cells/kg collected (×108/kg) | 6.98 (0.86–18.9) | Lymphoma: 5.37 (1.7–11.7) |
Multiple myeloma: 2.59 (0.73–12.0) | ||
CD34 + cells/kg collected (×106/kg) | 5.35 (2.4–12.8) | Lymphoma: 6.75 (1.9–34.6) |
Multiple myeloma: 5.98 (1.5–18.1) |
Allograft | Correlation (R Value) | p Value | |
---|---|---|---|
PD-L1+M-MDSC | PD-1+CD4+T | −0.192 | 0.126 |
PD-L1+M-MDSC | PD-1+CD8+T | −0.143 | 0.251 |
CD86+M-MDSC | CTLA-4+CD4+T | −0.388 | 0.001 |
CD86+M-MDSC | CTLA-4+CD8+T | −0.271 | <0.05 |
Galectin-9+M-MDSC | TIM-3+CD4+T | −0.341 | <0.01 |
Galectin-9+M-MDSC | TIM-3+CD8+T | −0.200 | 0.117 |
CD155+M-MDSC | TIGIT+CD4+T | −0.45 | <0.0001 |
CD155+M-MDSC | TIGIT+CD8+T | −0.205 | 0.098 |
HVEM+M-MDSC | BTLA+CD4+T | −0.358 | <0.01 |
HVEM+M-MDSC | BTLA+CD8+T | −0.327 | <0.05 |
Lymphoma autograft | |||
PD-L1+M-MDSC | PD-1+CD4+T | −0.350 | <0.05 |
PD-L1+M-MDSC | PD-1+CD8+T | −0.444 | <0.01 |
CD86+M-MDSC | CTLA-4+CD4+T | −0.379 | <0.05 |
CD86+M-MDSC | CTLA-4+CD8+T | −0.481 | <0.01 |
Galectin-9+M-MDSC | TIM-3+CD4+T | −0.733 | <0.001 |
Galectin-9+M-MDSC | TIM-3+CD8+T | −0.691 | <0.001 |
CD155+M-MDSC | TIGIT+CD4+T | −0.648 | <0.0001 |
CD155+M-MDSC | TIGIT+CD8+T | −0.440 | <0.01 |
HVEM+M-MDSC | BTLA+CD4+T | −0.128 | 0.471 |
HVEM+M-MDSC | BTLA+CD8+T | −0.211 | 0.23 |
MM autograft | |||
PD-L1+M-MDSC | PD-1+CD4+T | −0.547 | <0.0001 |
PD-L1+M-MDSC | PD-1+CD8+T | −0.531 | <0.0001 |
CD86+M-MDSC | CTLA-4+CD4+T | −0.497 | <0.0001 |
CD86+M-MDSC | CTLA-4+CD8+T | −0.295 | <0.05 |
Galectin-9+M-MDSC | TIM-3+CD4+T | −0.524 | <0.0001 |
Galectin-9+M-MDSC | TIM-3+CD8+T | −0.354 | <0.01 |
CD155+M-MDSC | TIGIT+CD4+T | −0.597 | <0.0001 |
CD155+M-MDSC | TIGIT+CD8+T | −0.528 | <0.0001 |
HVEM+M-MDSC | BTLA+CD4+T | −0.359 | <0.01 |
HVEM+M-MDSC | BTLA+CD8+T | −0.271 | <0.05 |
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Guan, Q.; Gilpin, S.G.; Doerksen, J.; Bath, L.; Lam, T.; Li, Y.; Lambert, P.; Wall, D.A. The Interactions of T Cells with Myeloid-Derived Suppressor Cells in Peripheral Blood Stem Cell Grafts. Cells 2024, 13, 1545. https://doi.org/10.3390/cells13181545
Guan Q, Gilpin SG, Doerksen J, Bath L, Lam T, Li Y, Lambert P, Wall DA. The Interactions of T Cells with Myeloid-Derived Suppressor Cells in Peripheral Blood Stem Cell Grafts. Cells. 2024; 13(18):1545. https://doi.org/10.3390/cells13181545
Chicago/Turabian StyleGuan, Qingdong, Scott G. Gilpin, James Doerksen, Lauren Bath, Tracy Lam, Yun Li, Pascal Lambert, and Donna A. Wall. 2024. "The Interactions of T Cells with Myeloid-Derived Suppressor Cells in Peripheral Blood Stem Cell Grafts" Cells 13, no. 18: 1545. https://doi.org/10.3390/cells13181545
APA StyleGuan, Q., Gilpin, S. G., Doerksen, J., Bath, L., Lam, T., Li, Y., Lambert, P., & Wall, D. A. (2024). The Interactions of T Cells with Myeloid-Derived Suppressor Cells in Peripheral Blood Stem Cell Grafts. Cells, 13(18), 1545. https://doi.org/10.3390/cells13181545