CEP55 Promotes Cell Motility via JAK2–STAT3–MMPs Cascade in Hepatocellular Carcinoma
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents and Antibodies
2.2. Gene Expression and Survival Analysis
2.3. Quantitative Real-Time PCR
2.4. Lentivirus Construction and Infection
- CEP55 shRNA1: 5′-ACCTCACGAATTGCTGAAC-3′; CEP55 shRNA2: 5′-GACTGAGAGACCAACTGAA-3′;
- CEP55 shRNA3: 5′-GCTCCAAACTGCTTCAACT-3′; CEP55 shRNA4: 5′-CTGAAAGATGCTCTGGAGA-3′; and
- JAK2 shRNA: 5′-GCAGAATTAGCAAACCTTATA-3′; STAT3 shRNA: 5′-TGTTCTCTATCAGCACAAT-3′.
2.5. Western Blot
2.6. Co-Immunoprecipitation (Co-IP) Assay
2.7. Cell Wound Scratch Assay
2.8. Trans-Well Assay
2.9. Statistical Analysis
3. Results
3.1. CEP55 Expression Is Upregulated in HCC Tissues and Cell Lines
3.2. Overexpression of CEP55 Is a Poor Prognostic Factor for HCC Patients
3.3. CEP55 Knockdown Suppresses the Motility Activity of HCC Cells
3.4. CEP55 Promotes Expression of MMPs in HCC Cells
3.5. CEP55 Stimulates the JAK2–STAT3–MMP Axis in HCC Cells
3.6. JAK2 or STAT3 Blocking Abrogates the Pro-Migration and Pro-Invasion Effects of CEP55
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Variable | CEP55 mRNA Expression # | p-Value (χ2) | |
---|---|---|---|
Low | High | ||
Gender | |||
Male | 86 | 75 | 0.1540 |
Female | 30 | 41 | |
Age (year) | |||
≤50 | 18 | 30 | 0.0739 |
>50 | 98 | 86 | |
AFP (ng/mL) | |||
≤200 | 101 | 69 | <0.0001 * |
>200 | 15 | 47 | |
Hepatitis status | |||
Negative | 55 | 47 | 0.3545 |
Positive | 61 | 69 | |
Vascular invasion | |||
No | 91 | 72 | 0.0095 * |
Yes | 25 | 44 | |
Histologic grade | |||
I | 19 | 5 | <0.0001 * |
II | 64 | 43 | |
III–IV | 33 | 68 | |
TNM stage | |||
I | 74 | 52 | 0.02 * |
II | 22 | 30 | |
III–IV | 20 | 32 |
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Li, M.; Gao, J.; Li, D.; Yin, Y. CEP55 Promotes Cell Motility via JAK2–STAT3–MMPs Cascade in Hepatocellular Carcinoma. Cells 2018, 7, 99. https://doi.org/10.3390/cells7080099
Li M, Gao J, Li D, Yin Y. CEP55 Promotes Cell Motility via JAK2–STAT3–MMPs Cascade in Hepatocellular Carcinoma. Cells. 2018; 7(8):99. https://doi.org/10.3390/cells7080099
Chicago/Turabian StyleLi, Minjing, Ju Gao, Defang Li, and Yancun Yin. 2018. "CEP55 Promotes Cell Motility via JAK2–STAT3–MMPs Cascade in Hepatocellular Carcinoma" Cells 7, no. 8: 99. https://doi.org/10.3390/cells7080099