MiR-21, MiR-29a, GATA4, and MEF2c Expression Changes in Endothelin-1 and Angiotensin II Cardiac Hypertrophy Stimulated Isl-1+Sca-1+c-kit+ Porcine Cardiac Progenitor Cells In Vitro
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolation of Porcine Cardiac Progenitor Cells from Heart Tissue
2.2. Characterization of pCPCs
2.2.1. Immunofluorescence Staining
2.2.2. Real-Time qPCR
2.2.3. Transfection with pMx-MGT
2.2.4. Induction of Hypertrophy in pCPC
2.3. Measurement of Hypertrophy-Induced Protein Expression and Cell Size
2.4. Quantification of mRNA and miRNA Gene Expression by Real-Time qPCR
2.5. Statistics
3. Results
3.1. Isolation and Characterizaton of Isl1+Sca1+ckit+ pCPC
3.2. Induction of Chemical Hypertrophy with Ang II and ET-1 Results in Hypertrophic Growth of Isl1+Sca1+ckit+ pCPC
3.3. Cardiac Reprogramming of pCPCs with pMx-MGT or Ang II Induced Hypertrophy Alone Leads to Increase of Intracellular MCP-1
3.4. Cx43 is Upregulated in pMx-MGT-Transfected and Ang II Stimulated Isl1+Sca1+ckit+ pCPCs
3.5. Increased Cellular BNP Expression Confirms Cardiac Hypertrophy
3.6. ET-1 and Ang II Induced Hypertrophy Leads to Overexpression of MEF2c and GATA4 in Isl1+Sca1+ckit+ pCPCs
3.7. miR-29a is Upregulated in Response to Ang II Stimulation
3.8. pMx-MGT-Transfected Ang II Stimulated Isl1+Sca1+ckit+ pCPCs Have a Significanly Higher miR-21 Gene Expression
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Gene | Forward Primer | Reverse Primer |
---|---|---|
ISL-1 | ACATGACGGTGGCTTACAGG | ATGTCACTCTGCAAGGCGAA |
SCA-1/Ly6a | AGCTCAGGGACTGGAGTGTT | ATCAGGGTAGGGGCAGGTAA |
KIT (c-kit) | GCCTGTGACTATCTGGGCTC | GCCTTATTCATGCCCGGAGA |
BNP | CGCAGTAGCATCTTCCAAGTC | ACCTCCTGAGCACATTGCAG |
ACTB (HK) | TCAACACCCCAGCCATGTAC | CTCCGGAGTCCATCACGATG |
Gene | Forward Primer | Reverse Primer |
---|---|---|
MEF2c | TAACATGCCGCCATCCGCCC | ATCCTCTCGGTCGCTGCCGT |
GATA4 | AGAAAACGGAAGCCCAAGAAC | CCACACTGCTGGAGTTGCTG |
miR-29a | CGGACCTAGCACCATCTGAA | miScript Universal Primer (Qiagen) |
miR-21 | CGTAGCTAGCTTATCAGACTG | miScript Universal Primer (Qiagen) |
ACTB (HK) | TCAACACCCCAGCCATGTAC | CTCCGGAGTCCATCACGATG |
let7a (HK) | GCAGTGAGGTAGTAGGTTGT | miScript Universal Primer (Qiagen) |
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Zlabinger, K.; Spannbauer, A.; Traxler, D.; Gugerell, A.; Lukovic, D.; Winkler, J.; Mester-Tonczar, J.; Podesser, B.; Gyöngyösi, M. MiR-21, MiR-29a, GATA4, and MEF2c Expression Changes in Endothelin-1 and Angiotensin II Cardiac Hypertrophy Stimulated Isl-1+Sca-1+c-kit+ Porcine Cardiac Progenitor Cells In Vitro. Cells 2019, 8, 1416. https://doi.org/10.3390/cells8111416
Zlabinger K, Spannbauer A, Traxler D, Gugerell A, Lukovic D, Winkler J, Mester-Tonczar J, Podesser B, Gyöngyösi M. MiR-21, MiR-29a, GATA4, and MEF2c Expression Changes in Endothelin-1 and Angiotensin II Cardiac Hypertrophy Stimulated Isl-1+Sca-1+c-kit+ Porcine Cardiac Progenitor Cells In Vitro. Cells. 2019; 8(11):1416. https://doi.org/10.3390/cells8111416
Chicago/Turabian StyleZlabinger, Katrin, Andreas Spannbauer, Denise Traxler, Alfred Gugerell, Dominika Lukovic, Johannes Winkler, Julia Mester-Tonczar, Bruno Podesser, and Mariann Gyöngyösi. 2019. "MiR-21, MiR-29a, GATA4, and MEF2c Expression Changes in Endothelin-1 and Angiotensin II Cardiac Hypertrophy Stimulated Isl-1+Sca-1+c-kit+ Porcine Cardiac Progenitor Cells In Vitro" Cells 8, no. 11: 1416. https://doi.org/10.3390/cells8111416
APA StyleZlabinger, K., Spannbauer, A., Traxler, D., Gugerell, A., Lukovic, D., Winkler, J., Mester-Tonczar, J., Podesser, B., & Gyöngyösi, M. (2019). MiR-21, MiR-29a, GATA4, and MEF2c Expression Changes in Endothelin-1 and Angiotensin II Cardiac Hypertrophy Stimulated Isl-1+Sca-1+c-kit+ Porcine Cardiac Progenitor Cells In Vitro. Cells, 8(11), 1416. https://doi.org/10.3390/cells8111416