H2O2 as a Feedback Signal on Dual-Located WHIRLY1 Associates with Leaf Senescence in Arabidopsis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Culture Conditions
2.2. Chlorophyll Fluorescence and Concentration Measurement
2.3. Measurement of H2O2 Content in Rosette Leaves
2.4. Staining of Hydrogen Peroxide
2.5. Quantitative RT-PCR Analysis (qRT-PCR)
2.6. In Vitro DNA-Binding Assays
2.7. Isolation and Detection of Plastid and Nuclear Proteins
2.8. ChIP-qPCR Assay
2.9. Statistical Analysis
3. Results
3.1. Ectopic Expression of a Plastid Isoform WHY1 Causes a Strong Leaf Senescence
3.2. Allocation of WHY1 into Plastid-Enhanced Production of Reactive Oxygen Species (ROS)
3.3. PRX33 and PRX39 were Downstream of WHY1 but with no Obvious Involvement in WHY1-Mediated ROS Pathway
3.4. H2O2 Treatments Altered WHY1 Protein Distribution Between Plastids and the Nucleus but Not Its mRNA Levels
3.5. H2O2 Induces the Enrichment of H3K9ac and RNAP II at WRKY53 Promoter Region
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Lin, W.; Huang, D.; Shi, X.; Deng, B.; Ren, Y.; Lin, W.; Miao, Y. H2O2 as a Feedback Signal on Dual-Located WHIRLY1 Associates with Leaf Senescence in Arabidopsis. Cells 2019, 8, 1585. https://doi.org/10.3390/cells8121585
Lin W, Huang D, Shi X, Deng B, Ren Y, Lin W, Miao Y. H2O2 as a Feedback Signal on Dual-Located WHIRLY1 Associates with Leaf Senescence in Arabidopsis. Cells. 2019; 8(12):1585. https://doi.org/10.3390/cells8121585
Chicago/Turabian StyleLin, Wenfang, Dongmei Huang, Ximiao Shi, Ban Deng, Yujun Ren, Wenxiong Lin, and Ying Miao. 2019. "H2O2 as a Feedback Signal on Dual-Located WHIRLY1 Associates with Leaf Senescence in Arabidopsis" Cells 8, no. 12: 1585. https://doi.org/10.3390/cells8121585
APA StyleLin, W., Huang, D., Shi, X., Deng, B., Ren, Y., Lin, W., & Miao, Y. (2019). H2O2 as a Feedback Signal on Dual-Located WHIRLY1 Associates with Leaf Senescence in Arabidopsis. Cells, 8(12), 1585. https://doi.org/10.3390/cells8121585