Activation of Cryptic 3′ Splice-Sites by SRSF2 Contributes to Cassette Exon Skipping
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plasmids Construction
2.2. Cell Culture, Transfection and RT-PCR
2.3. Knockdown of SRSF2 with Lentivirus-Mediated shRNA
3. Results
3.1. SRSF2 Promotes Cryptic 3′AG′ Activation
3.2. Deletion of 3′AG′ Region Induces SRSF2-Dependent Usage of Alternate 3′AG′
3.3. Multiple SRSF2-Binding Sequences and Clusters Are Necessary for 3′AG′ Activation by SRSF2
3.4. 5′Splice-Site Mutations do not Affect SRSF2-Mediated 3′AG′ Activation
4. Discussion
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Moon, H.; Jang, H.N.; Liu, Y.; Choi, N.; Oh, J.; Ha, J.; Zheng, X.; Shen, H. Activation of Cryptic 3′ Splice-Sites by SRSF2 Contributes to Cassette Exon Skipping. Cells 2019, 8, 696. https://doi.org/10.3390/cells8070696
Moon H, Jang HN, Liu Y, Choi N, Oh J, Ha J, Zheng X, Shen H. Activation of Cryptic 3′ Splice-Sites by SRSF2 Contributes to Cassette Exon Skipping. Cells. 2019; 8(7):696. https://doi.org/10.3390/cells8070696
Chicago/Turabian StyleMoon, Heegyum, Ha Na Jang, Yongchao Liu, Namjeong Choi, Jagyeong Oh, Jiyeon Ha, Xuexiu Zheng, and Haihong Shen. 2019. "Activation of Cryptic 3′ Splice-Sites by SRSF2 Contributes to Cassette Exon Skipping" Cells 8, no. 7: 696. https://doi.org/10.3390/cells8070696