Exposure to Ionizing Radiation Triggers Prolonged Changes in Circular RNA Abundance in the Embryonic Mouse Brain and Primary Neurons
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Cell Culture and Transfection
2.3. X-Irradiation
2.4. RNA Extraction, cDNA Synthesis, and RT-qPCR
2.5. Ribonuclease-R (RNaseR) Treatment
2.6. Actinomycin-D Treatment
2.7. Statistical Analysis
3. Results
3.1. Validation of Predicted Non-Coding Circular Transcripts of P53-Regulated Genes
3.2. Circular Transcripts are More Stable Compared to Linear Cognates in Primary Cortical Neurons
3.3. Tissue-Specific Presence of Circular Transcripts is Independent of Their Host mRNAs and is Enriched in the Brain
3.4. Steady-State Levels of circRNA are Enriched During Pre- and Post-Natal Brain Development and Neuronal Maturation
3.5. Prolonged Upregulation of circRNA Transcript Levels in the Embryonic Brain and Primary Cortical Neurons
3.6. Long-Term Induction of Pvt1 circRNA Abundance in Mouse Blood after Whole Body Irradiation
3.7. Expression of Pvt1 circRNAs in Neuro-2a Cells Is Independent of the circRNA Biogenesis Regulator Quaking
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Mbouombouo Mfossa, A.C.; Thekkekara Puthenparampil, H.; Inalegwu, A.; Coolkens, A.; Baatout, S.; Benotmane, M.A.; Huylebroeck, D.; Quintens, R. Exposure to Ionizing Radiation Triggers Prolonged Changes in Circular RNA Abundance in the Embryonic Mouse Brain and Primary Neurons. Cells 2019, 8, 778. https://doi.org/10.3390/cells8080778
Mbouombouo Mfossa AC, Thekkekara Puthenparampil H, Inalegwu A, Coolkens A, Baatout S, Benotmane MA, Huylebroeck D, Quintens R. Exposure to Ionizing Radiation Triggers Prolonged Changes in Circular RNA Abundance in the Embryonic Mouse Brain and Primary Neurons. Cells. 2019; 8(8):778. https://doi.org/10.3390/cells8080778
Chicago/Turabian StyleMbouombouo Mfossa, André Claude, Helene Thekkekara Puthenparampil, Auchi Inalegwu, Amelie Coolkens, Sarah Baatout, Mohammed A. Benotmane, Danny Huylebroeck, and Roel Quintens. 2019. "Exposure to Ionizing Radiation Triggers Prolonged Changes in Circular RNA Abundance in the Embryonic Mouse Brain and Primary Neurons" Cells 8, no. 8: 778. https://doi.org/10.3390/cells8080778