N-Acetylcysteine Serves as Substrate of 3-Mercaptopyruvate Sulfurtransferase and Stimulates Sulfide Metabolism in Colon Cancer Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of Sulfide Stock Solutions
2.3. Cell Culture and Isolation of Mitochondria
2.4. Human MST Expression and Purification
2.5. SQR Activity Assay in Mitochondrial Preparations
2.6. MST Activity Assay in Cell Lysates
2.7. Recombinant Human MST Activity Assay
2.8. Evaluation of Mitochondrial Content by the Citrate Synthase Assay
2.9. Immunoblotting Assays
2.10. NAC Quantification by Reverse Phase High Performance Liquid Chromatography (RP-HPLC)
3. Results
3.1. Effect of NAC on Expression Levels of H2S Metabolism Enzymes
3.2. Effect of NAC on MST Activity
3.3. Effect of NAC on SQR Activity
3.4. Effect of NAC on Mitochondrial Mass
3.5. Kinetics of H2S Production by Recombinant Human MST with NAC as a Substrate
3.6. HPLC Quantification of Intracellular NAC
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
References
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KM (A) | Vmax (A) | Vmax/KM (A) | KM (3MP) | Vmax (3MP) | Vmax/KM (3MP) | |
---|---|---|---|---|---|---|
mM | RFU·min−1 | RFU·min−1·mM−1 | mM | RFU·min−1 | RFU·min−1·mM−1 | |
Cys | 6.0 ± 1.0 | 15,136 ± 839 | 2523 | 0.57 ± 0.04 | 81,560 ± 3972 | 143,088 |
NAC | 11.4 ± 1.0 | 37,178 ± 1442 | 3261 | 0.49 ± 0.02 | 95,396 ± 2487 | 194,686 |
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Zuhra, K.; Tomé, C.S.; Masi, L.; Giardina, G.; Paulini, G.; Malagrinò, F.; Forte, E.; Vicente, J.B.; Giuffrè, A. N-Acetylcysteine Serves as Substrate of 3-Mercaptopyruvate Sulfurtransferase and Stimulates Sulfide Metabolism in Colon Cancer Cells. Cells 2019, 8, 828. https://doi.org/10.3390/cells8080828
Zuhra K, Tomé CS, Masi L, Giardina G, Paulini G, Malagrinò F, Forte E, Vicente JB, Giuffrè A. N-Acetylcysteine Serves as Substrate of 3-Mercaptopyruvate Sulfurtransferase and Stimulates Sulfide Metabolism in Colon Cancer Cells. Cells. 2019; 8(8):828. https://doi.org/10.3390/cells8080828
Chicago/Turabian StyleZuhra, Karim, Catarina S. Tomé, Letizia Masi, Giorgio Giardina, Giulia Paulini, Francesca Malagrinò, Elena Forte, João B. Vicente, and Alessandro Giuffrè. 2019. "N-Acetylcysteine Serves as Substrate of 3-Mercaptopyruvate Sulfurtransferase and Stimulates Sulfide Metabolism in Colon Cancer Cells" Cells 8, no. 8: 828. https://doi.org/10.3390/cells8080828