Heat Stress-Induced Multiple Multipolar Divisions of Human Cancer Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Heat Stress Treatment
2.3. MTT Assay
2.4. Flow Cytometry
2.5. Long-Term Live Imaging
2.6. Immunofluorescence Assay
2.7. Statistical Analysis
3. Results
3.1. Biological Responses of Human Cancer Cells to Heat Stress
3.2. Multipolar Divisions Induced by Heat Stress
3.2.1. The Route of Mitotic Slippage
3.2.2. The Route of Cytokinesis Failure
3.2.3. The Route of Cell Fusion
3.2.4. The Route of Centrosome Aberration
3.2.5. Incomes of Multiple Multipolar Divisions Induced by Heat Stress
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Chen, S.; Liu, M.; Huang, H.; Li, B.; Zhao, H.; Feng, X.-Q.; Zhao, H.-P. Heat Stress-Induced Multiple Multipolar Divisions of Human Cancer Cells. Cells 2019, 8, 888. https://doi.org/10.3390/cells8080888
Chen S, Liu M, Huang H, Li B, Zhao H, Feng X-Q, Zhao H-P. Heat Stress-Induced Multiple Multipolar Divisions of Human Cancer Cells. Cells. 2019; 8(8):888. https://doi.org/10.3390/cells8080888
Chicago/Turabian StyleChen, Shaoyong, Mingyue Liu, Huiming Huang, Bo Li, Hucheng Zhao, Xi-Qiao Feng, and Hong-Ping Zhao. 2019. "Heat Stress-Induced Multiple Multipolar Divisions of Human Cancer Cells" Cells 8, no. 8: 888. https://doi.org/10.3390/cells8080888