Autophagy Stimulation as a Potential Strategy Against Intestinal Fibrosis
Abstract
:1. Introduction
2. Methods
2.1. Heterotopic Transplant Model of Intestinal Fibrosis
2.2. Primary Intestinal Fibroblasts Isolation and Culture
2.3. RNA Extraction and Quantitative PCR
2.4. Western Blot
2.5. Sirius Red Staining
2.6. Statistical Analysis
3. Results
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Cosin-Roger, J.; Canet, F.; Macias-Ceja, D.C.; Gisbert-Ferrándiz, L.; Ortiz-Masiá, D.; Esplugues, J.V.; Alós, R.; Navarro, F.; Barrachina, M.D.; Calatayud, S. Autophagy Stimulation as a Potential Strategy Against Intestinal Fibrosis. Cells 2019, 8, 1078. https://doi.org/10.3390/cells8091078
Cosin-Roger J, Canet F, Macias-Ceja DC, Gisbert-Ferrándiz L, Ortiz-Masiá D, Esplugues JV, Alós R, Navarro F, Barrachina MD, Calatayud S. Autophagy Stimulation as a Potential Strategy Against Intestinal Fibrosis. Cells. 2019; 8(9):1078. https://doi.org/10.3390/cells8091078
Chicago/Turabian StyleCosin-Roger, Jesus, Francisco Canet, Dulce C. Macias-Ceja, Laura Gisbert-Ferrándiz, Dolores Ortiz-Masiá, Juan V. Esplugues, Rafael Alós, Francisco Navarro, María D. Barrachina, and Sara Calatayud. 2019. "Autophagy Stimulation as a Potential Strategy Against Intestinal Fibrosis" Cells 8, no. 9: 1078. https://doi.org/10.3390/cells8091078