JNK1 Induces Notch1 Expression to Regulate Genes Governing Photoreceptor Production
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mice
2.2. Cell Culture
2.3. Antibodies and Reagents
2.4. Real-Time PCR
- β-actin: sense (AAAGACCTGTACGCCAACAC),
- antisense (GTCATACTCCTGCTTGCTGAT);
- c-Jun: sense (GCAAGCCCTGAAGGAAGAG),
- antisense (GTCAT ACTCCTGCTTGCTGAT);
- Neurod1: sense (GACGGGGTCCCAAAAAGAAAA),
- antisense (GCCAAGCGCAGTGTCTCTATT);
- Crx: sense (GTTCAAGAATCGTAGGGCGAA),
- antisense (TGAGATGCCCAAAGGATCTGT);
- Rxrg: sense (CATGAGCCCTTCAGTAGCCTT),
- antisense (CGGAGAGCCAAGAGCATTGAG);
- Notch1: sense (CCGTGTAAGAATGCTGGAACG),
- antisense (AGCGACAGATGTATGAAGACTCA);
- Opn1sw: sense (CAGCCTTCATGGGATTTGTCT),
- antisense (CAAAGAGGAAGTATCCGTGACAG);
- Opn1mw: sense (ATGGCCCAAAGGCTTACAGG),
- antisense (AAGGGACCTTTGGTGCTGTT);
- Notch2: sense (GAGAAAAACCGCTGTCAGAATGG),
- antisense (GGTGGAGTATTGGCAGTCCTC);
- Notch3: sense (TGCCAGAGTTCAGTGGTGG),
- antisense (CACAGGCAAATCGGCCATC);
- Wnt2b: sense (CCGACGTGTCCCCATCTTC),
- antisense (GCCCCTATGTACCACCAGGA);
- Wnt5a: sense (CAACTGGCAGGACTTTCTCAA),
- antisense (CATCTCCGATGCCGGAACT);
- Rho: sense (CCCTTCTCCAACGTCACAGG),
- antisense (TGAGGAAGTTGATGGGGAAGC);
- Nrl: sense (TCCCAGTCCCTTGGCTATGG),
- antisense (CACCGAGCTGTATGGTGTG);
- Onecut2: sense (GGCTTCCGTCCATGAACAAC),
- antisense (CGAAATTGGGGCTGAGCATTTT);
- Chx10: sense (CTGAGCAAGCCCAAATCCGA),
- antisense (CGCAGCTAACAAATGCCCAG);
- Stx1a: sense (AGAGATCCGGGGCTTTATTGA),
- antisense (AATGCTCTTTAGCTTGGAGCG);
- Vim: sense (CGGCTGCGAGAGAAATTGC),
- antisense (CCACTTTCCGTTCAAGGTCAAG);
- Sox9: sense (CGGAACAGACTCACATCTCTCC),
- antisense (GCTTGCACGTCGGTTTTGG);
- Rbpms: sense (CCCGTAGGCTTTGTCAGTTTT),
- antisense (GTAAAGTGCAGGTACTGTGAGC);
- Prkca: sense (GTTTACCCGGCCAACGACT),
- antisense (GGGCGATGAATTTGTGGTCTT);
2.5. Western Blot
2.6. Subcellular Fractionation
2.7. Histology and Immunohistochemistry
2.8. Immunofluorescence
2.9. Chromatin Immunoprecipitation Assay
2.10. Plasmid Constructs
2.11. Transfection and Luciferase Assay
2.12. Cell Isolation and Flow Cytometry
2.13. Electroretinogram (ERG) Recording
2.14. Statistical Analysis
3. Results
3.1. Knocking Out JNK1 Promoted Retinogenesis
3.2. The Number of Photoreceptors Increased in Jnk1 KO Mice
3.3. Enhanced ERG Responses in the Jnk1 KO Mice
3.4. Light Activated JNK1, but Not JNK2, in 661w Cells
3.5. JNK1 Attenuated the Transcription of Genes Important for Photoreceptors
3.6. The Suppressive Effect of JNK1 Was Dependent on Notch1
3.7. JNK1 Regulated Notch1 Transcription via Activating c-Jun
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Pan, M.; Hu, H.; Wang, R.; Zhou, Y.; Zhang, L.; Wang, C.; Wang, Q. JNK1 Induces Notch1 Expression to Regulate Genes Governing Photoreceptor Production. Cells 2019, 8, 970. https://doi.org/10.3390/cells8090970
Pan M, Hu H, Wang R, Zhou Y, Zhang L, Wang C, Wang Q. JNK1 Induces Notch1 Expression to Regulate Genes Governing Photoreceptor Production. Cells. 2019; 8(9):970. https://doi.org/10.3390/cells8090970
Chicago/Turabian StylePan, Mingyu, Haiyang Hu, Rui Wang, Yi Zhou, Lele Zhang, Chen Wang, and Quanyi Wang. 2019. "JNK1 Induces Notch1 Expression to Regulate Genes Governing Photoreceptor Production" Cells 8, no. 9: 970. https://doi.org/10.3390/cells8090970