IGF-1 Signalling Regulates Mitochondria Dynamics and Turnover through a Conserved GSK-3β–Nrf2–BNIP3 Pathway
Abstract
:1. Introduction
2. Materials and Methods
2.1. List of Abbreviations
2.2. Antibodies
2.3. Cell Lines and Cell Culture
2.4. SiRNA Transfection
2.5. Plasmid Transfection
2.6. Immunofluorescence
2.7. Cell Lysis, SDS-PAGE and Western Blotting
2.8. Subcellular Fractionation
2.9. Quantitative Real-Time PCR
2.10. Flow Cytometry
2.11. Analysis of Transcription Factor Binding Sites in the BNIP3 Promoter Region
3. Results
3.1. Nrf2 Is Required for IGF-1-Mediated Induction of the Mitophagy Receptor BNIP3
3.2. IGF-1 Induced BNIP3 through an Inhibitory Phosphorylation of GSK-3β
3.3. Nrf2 Induced BNIP3 Expression through HIF-1α and NRF1
3.4. The IGF-1-Nrf2-BNIP3 Pathway Regulates Mitochondrial Dynamics and Cellular Capacity for Autophagosomal Turnover in Response to Metabolic and Mitochondrial Stress
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Riis, S.; Murray, J.B.; O’Connor, R. IGF-1 Signalling Regulates Mitochondria Dynamics and Turnover through a Conserved GSK-3β–Nrf2–BNIP3 Pathway. Cells 2020, 9, 147. https://doi.org/10.3390/cells9010147
Riis S, Murray JB, O’Connor R. IGF-1 Signalling Regulates Mitochondria Dynamics and Turnover through a Conserved GSK-3β–Nrf2–BNIP3 Pathway. Cells. 2020; 9(1):147. https://doi.org/10.3390/cells9010147
Chicago/Turabian StyleRiis, Sarah, Joss B. Murray, and Rosemary O’Connor. 2020. "IGF-1 Signalling Regulates Mitochondria Dynamics and Turnover through a Conserved GSK-3β–Nrf2–BNIP3 Pathway" Cells 9, no. 1: 147. https://doi.org/10.3390/cells9010147