Next Article in Journal
Human and Mouse TRPA1 Are Heat and Cold Sensors Differentially Tuned by Voltage
Next Article in Special Issue
Gold Nanoparticles Modulate BCG-Induced Innate Immune Memory in Human Monocytes by Shifting the Memory Response towards Tolerance
Previous Article in Journal
Dampened Slow Oscillation Connectivity Anticipates Amyloid Deposition in the PS2APP Mouse Model of Alzheimer’s Disease
Previous Article in Special Issue
Size Dependency of Circulation and Biodistribution of Biomimetic Nanoparticles: Red Blood Cell Membrane-Coated Nanoparticles
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Cells
Volume 9
Issue 1
10.3390/cells9010056
cells-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

CXCL5 Modified Nanoparticle Surface Improves CXCR2+ Cell Selective Internalization

by
Roberta Cagliani
1,2,
Francesca Gatto
1,
Giulia Cibecchini
1,2,
Roberto Marotta
3,
Federico Catalano
3,
Paola Sanchez-Moreno
1,
Pier Paolo Pompa
1 and
Giuseppe Bardi
1,*
1
Nanobiointeractions & Nanodiagnostics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy
2
Department of Chemistry and Industrial Chemistry, University of Genova, Via Dodecaneso 31, 16146 Genova, Italy
3
Electron Microscopy Laboratory, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy
*
Author to whom correspondence should be addressed.
Cells 2020, 9(1), 56; https://doi.org/10.3390/cells9010056
Submission received: 27 November 2019 / Revised: 17 December 2019 / Accepted: 24 December 2019 / Published: 24 December 2019
(This article belongs to the Special Issue The Interaction of Biomedical Nanoparticles with the Immune System)
Browse Figures
Versions Notes

Abstract

Driving nanomaterials to specific cell populations is still a major challenge for different biomedical applications. Several strategies to improve cell binding and uptake have been tried thus far by intrinsic material modifications or decoration with active molecules onto their surface. In the present work, we covalently bound the chemokine CXCL5 on fluorescently labeled amino-functionalized SiO2 nanoparticles to precisely targeting CXCR2+ immune cells. We synthesized and precisely characterized the physicochemical features of the modified particles. The presence of CXCL5 on the surface was detected by z-potential variation and CXCL5-specific electron microscopy immunogold labeling. CXCL5-amino SiO2 nanoparticle cell binding and internalization performances were analyzed in CXCR2+ THP-1 cells by flow cytometry and confocal microscopy. We showed improved internalization of the chemokine modified particles in the absence or the presence of serum. This internalization was reduced by cell pre-treatment with free CXCL5. Furthermore, we demonstrated CXCR2+ cell preferential targeting by comparing particle uptake in THP-1 vs. low-CXCR2 expressing HeLa cells. Our results provide the proof of principle that chemokine decorated nanomaterials enhance uptake and allow precise cell subset localization. The possibility to aim at selective chemokine receptor-expressing cells can be beneficial for the diverse pathological conditions involving immune reactions.
Keywords: chemokines; chemokine receptors; immune cells; nanoparticles; surface chemistry chemokines; chemokine receptors; immune cells; nanoparticles; surface chemistry

Share and Cite

MDPI and ACS Style

Cagliani, R.; Gatto, F.; Cibecchini, G.; Marotta, R.; Catalano, F.; Sanchez-Moreno, P.; Pompa, P.P.; Bardi, G. CXCL5 Modified Nanoparticle Surface Improves CXCR2+ Cell Selective Internalization. Cells 2020, 9, 56. https://doi.org/10.3390/cells9010056

AMA Style

Cagliani R, Gatto F, Cibecchini G, Marotta R, Catalano F, Sanchez-Moreno P, Pompa PP, Bardi G. CXCL5 Modified Nanoparticle Surface Improves CXCR2+ Cell Selective Internalization. Cells. 2020; 9(1):56. https://doi.org/10.3390/cells9010056

Chicago/Turabian Style

Cagliani, Roberta, Francesca Gatto, Giulia Cibecchini, Roberto Marotta, Federico Catalano, Paola Sanchez-Moreno, Pier Paolo Pompa, and Giuseppe Bardi. 2020. "CXCL5 Modified Nanoparticle Surface Improves CXCR2+ Cell Selective Internalization" Cells 9, no. 1: 56. https://doi.org/10.3390/cells9010056

APA Style

Cagliani, R., Gatto, F., Cibecchini, G., Marotta, R., Catalano, F., Sanchez-Moreno, P., Pompa, P. P., & Bardi, G. (2020). CXCL5 Modified Nanoparticle Surface Improves CXCR2+ Cell Selective Internalization. Cells, 9(1), 56. https://doi.org/10.3390/cells9010056

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop