Circular RNA Encoded Amyloid Beta peptides—A Novel Putative Player in Alzheimer’s Disease
Abstract
:1. Introduction
2. Materials and Methods
2.1. circAβ-a Identification via RT-PCR and Sequencing
2.2. Plasmid Construction
2.3. Cell Culture and Plasmid DNA Transfection
2.4. Total RNA Isolation and qRT-qPCR
2.5. Northern Blot Hybridization
2.6. Western Blot Analysis
2.7. Immunoprecipitation–Mass Spectrometry (IP-MS) of circAβ-a Derived Protein
2.8. Immunoprecipitation-Western Blotting (IP-WB) of Aβ Peptides
2.9. Internal Ribosome Entry Site (IRES) Analysis
3. Results
3.1. Presence of circAβ-a in the Brain of AD Patients and Non-Dementia Controls
3.2. Presence of the Additional APP hnRNA-Derived Circular RNAs in Human Brain
3.3. Intron-Mediated Enhancement (IME) Promotes circAβ-a Overexpression in Human Cells
3.4. circAβ-a can be Translated into an Aβ-Related Protein in Human Cells
3.5. Expression of circAβ-a Translated Protein (Aβ175) in Human Brain
3.6. Aβ175 Generates Aβ Peptides in HEK293 Cells
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Mo, D.; Li, X.; Raabe, C.A.; Rozhdestvensky, T.S.; Skryabin, B.V.; Brosius, J. Circular RNA Encoded Amyloid Beta peptides—A Novel Putative Player in Alzheimer’s Disease. Cells 2020, 9, 2196. https://doi.org/10.3390/cells9102196
Mo D, Li X, Raabe CA, Rozhdestvensky TS, Skryabin BV, Brosius J. Circular RNA Encoded Amyloid Beta peptides—A Novel Putative Player in Alzheimer’s Disease. Cells. 2020; 9(10):2196. https://doi.org/10.3390/cells9102196
Chicago/Turabian StyleMo, Dingding, Xinping Li, Carsten A. Raabe, Timofey S. Rozhdestvensky, Boris V. Skryabin, and Juergen Brosius. 2020. "Circular RNA Encoded Amyloid Beta peptides—A Novel Putative Player in Alzheimer’s Disease" Cells 9, no. 10: 2196. https://doi.org/10.3390/cells9102196