Hypoxia Promotes Mitochondrial Complex I Abundance via HIF-1α in Complex III and Complex IV Deficient Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Normoxic and Hypoxic Conditions
2.3. Doxycycline Treatment to Deplete Mitochondrial Respiratory Complexes
2.4. Determination of Mitochondrial ROS Production Using Redox Sensitive GFP
2.5. Blue Native Gel Electrophoresis, SDS-Polyacrylamide Gel Electrophoresis and Western Blotting
2.6. HIF-1α SiRNA Silencing
2.7. Statistical Analysis
3. Results
3.1. Stabilization of CI by Different Oxygen Concentrations in CIII and CIV Deficient Cells
3.2. Hypoxia Increased the Levels of CI in CYTB Cybrids and Caused Redistribution of CI in COX1 Cybrids
3.3. De Novo Assembly of CI after Depletion of Mitochondrial Encoded Subunits Is Faster in Hypoxia
3.4. Hypoxia Did Not Increase ROS in RISP and COX10 Fibroblasts
3.5. Dependence of HIF-1α on the Stability of CI and Respiratory Complexes
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Saldana-Caboverde, A.; Nissanka, N.; Garcia, S.; Lombès, A.; Diaz, F. Hypoxia Promotes Mitochondrial Complex I Abundance via HIF-1α in Complex III and Complex IV Deficient Cells. Cells 2020, 9, 2197. https://doi.org/10.3390/cells9102197
Saldana-Caboverde A, Nissanka N, Garcia S, Lombès A, Diaz F. Hypoxia Promotes Mitochondrial Complex I Abundance via HIF-1α in Complex III and Complex IV Deficient Cells. Cells. 2020; 9(10):2197. https://doi.org/10.3390/cells9102197
Chicago/Turabian StyleSaldana-Caboverde, Amy, Nadee Nissanka, Sofia Garcia, Anne Lombès, and Francisca Diaz. 2020. "Hypoxia Promotes Mitochondrial Complex I Abundance via HIF-1α in Complex III and Complex IV Deficient Cells" Cells 9, no. 10: 2197. https://doi.org/10.3390/cells9102197