MRE11 Is Crucial for Malaria Parasite Transmission and Its Absence Affects Expression of Interconnected Networks of Key Genes Essential for Life
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics
2.2. Animals
2.3. Generation of Transgenic Parasites
2.4. Parasite Genotyping and Western Blotting
2.5. RNA-Seq Transcriptome Sequencing
2.6. Phenotypic Analysis
2.7. Electron Microscopy
2.8. Statistical Analyses
2.9. Data Availability
3. Results
3.1. MRE11-GFP Has a Nuclear Location and Is Female Cell Lineage Specific
3.2. MRE11 Is Required for Oocyst Maturation and Sporogony in the Mosquito
3.3. Ultrastructure Analysis Reveals Degenerative Cytoplasm and Complete Lack of Sporozoite Formation in Δmre11 Parasites
3.4. Absence of PbMRE11 Results in Transcriptional Downregulation of Key Genes Essential to All Eukaryotic Life
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Guttery, D.S.; Ramaprasad, A.; Ferguson, D.J.P.; Zeeshan, M.; Pandey, R.; Brady, D.; Holder, A.A.; Pain, A.; Tewari, R. MRE11 Is Crucial for Malaria Parasite Transmission and Its Absence Affects Expression of Interconnected Networks of Key Genes Essential for Life. Cells 2020, 9, 2590. https://doi.org/10.3390/cells9122590
Guttery DS, Ramaprasad A, Ferguson DJP, Zeeshan M, Pandey R, Brady D, Holder AA, Pain A, Tewari R. MRE11 Is Crucial for Malaria Parasite Transmission and Its Absence Affects Expression of Interconnected Networks of Key Genes Essential for Life. Cells. 2020; 9(12):2590. https://doi.org/10.3390/cells9122590
Chicago/Turabian StyleGuttery, David S., Abhinay Ramaprasad, David J. P. Ferguson, Mohammad Zeeshan, Rajan Pandey, Declan Brady, Anthony A. Holder, Arnab Pain, and Rita Tewari. 2020. "MRE11 Is Crucial for Malaria Parasite Transmission and Its Absence Affects Expression of Interconnected Networks of Key Genes Essential for Life" Cells 9, no. 12: 2590. https://doi.org/10.3390/cells9122590