Adenosinergic System Involvement in Ischemic Stroke Patients’ Lymphocytes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ischemic Stroke Patients and Healthy Subjects
2.2. Sample Collection and Human Lymphocyte Preparation
2.3. Real-Time Quantitative Polymerase Chain Reaction Assays
2.4. Saturation Binding Assays to A1, A2A, A2B, and A3ARs
2.5. Flow Cytometry Analysis
2.6. Statistical Analysis
3. Results
3.1. A2AAR mRNA Expression is Up-Regulated in Lymphocytes of Ischemic Stroke Patients
3.2. A2AAR Increase in Density and Affinity in Lymphocytes of Ischemic Stroke Patients
3.3. CD4 and CD8 Expression on Lymphocytes of Ischemic Stroke Patients
3.4. CD73 and CD39 Expression on Lymphocytes of Ischemic Stroke Patients
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Ischemic Stroke Patients | n = 71 |
Demographic features | |
N° female/male | 35/36 |
Age | 78.31 ± 13.13 |
Hypertension | 48 |
Diabetes | 12 |
Smoke | 10 |
Atrial fibrillation | 26 |
OCSP patients classification | |
TACI | 19 |
PACI | 36 |
POCI | 8 |
LACI | 8 |
NIHSS | |
Slight impairment (1–5) | 26 |
Moderate impairment (6–14) | 26 |
Severe impairment (15–25) | 19 |
Very severe impairment (>25) | 0 |
TOAST Classification | |
LAA | 20 |
CE | 34 |
SVA | 7 |
Other | 1 |
Undetermined | 9 |
Healthy Subjects | n = 70 |
Demographic features | |
N° female/male | 31/39 |
Age | 61.24 ± 12.17 |
A1ARs | A2AARs | A2BARs | A3ARs | |
---|---|---|---|---|
Healthy subjects (n = 50) | ||||
KD (nM) | 1.74 ± 0.11 | 1.48 ± 0.10 | 2.11 ± 0.20 | 1.87 ± 0.14 |
Bmax (fmol/mg protein) | 52 ± 4 | 63 ± 5 | 53 ± 5 | 76 ± 8 |
Ischemic stroke patients (n = 50) | ||||
KD (nM) | 1.58 ± 0.12 | 0.97 ± 0.04** | 2.34 ± 0.16 | 1.91 ± 0.11 |
Bmax (fmol/mg protein) | 45 ± 3 | 168 ± 14** | 58 ± 6 | 87 ± 9 |
CD39 (%) | CD73 (%) | |||
---|---|---|---|---|
Healthy Subjects | Ischemic Stroke Patients | Healthy Subjects | Ischemic Stroke Patients | |
All lymphocytes | 14.82 ± 0.66 | 16.22 ± 1.16 | 10.04 ± 1.10 | 6.98 ± 0.77* |
CD4+ lymphocytes | 8.56 ± 0.74 | 11.35 ± 1.29 | 2.96 ± 0.45 | 1.47 ± 1.10* |
CD8+ lymphocytes | 4.09 ± 0.53 | 6.50 ± 0.90 | 21.04 ± 2.54 | 10.54 ± 1.48** |
CD39 (MFI) | CD73 (MFI) | |||
---|---|---|---|---|
Healthy Subjects | Ischemic Stroke Patients | Healthy Subjects | Ischemic Stroke Patients | |
All lymphocytes | 5389 ± 515 | 4502 ± 416 | 10084 ± 588 | 11436 ± 986 |
CD4+ lymphocytes | 4783 ± 258 | 4715 ± 380 | 6983 ± 234 | 7058 ± 396 |
CD8+ lymphocytes | 2640 ± 208 | 2357 ± 212 | 5197 ± 246 | 4239 ± 184** |
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Pasquini, S.; Vincenzi, F.; Casetta, I.; Laudisi, M.; Merighi, S.; Gessi, S.; Borea, P.A.; Varani, K. Adenosinergic System Involvement in Ischemic Stroke Patients’ Lymphocytes. Cells 2020, 9, 1072. https://doi.org/10.3390/cells9051072
Pasquini S, Vincenzi F, Casetta I, Laudisi M, Merighi S, Gessi S, Borea PA, Varani K. Adenosinergic System Involvement in Ischemic Stroke Patients’ Lymphocytes. Cells. 2020; 9(5):1072. https://doi.org/10.3390/cells9051072
Chicago/Turabian StylePasquini, Silvia, Fabrizio Vincenzi, Ilaria Casetta, Michele Laudisi, Stefania Merighi, Stefania Gessi, Pier Andrea Borea, and Katia Varani. 2020. "Adenosinergic System Involvement in Ischemic Stroke Patients’ Lymphocytes" Cells 9, no. 5: 1072. https://doi.org/10.3390/cells9051072