Multi-Omics Analysis of Diabetic Heart Disease in the db/db Model Reveals Potential Targets for Treatment by a Longevity-Associated Gene
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics
2.2. Viral Vector
2.3. In Vivo Protocol
2.4. Next-Generation RNA Sequencing
2.5. RNA Isolation and RT-qPCR
2.6. Protein Extraction and Western Blotting
2.7. Untargeted Metabolomics–Ultra-High-Performance Liquid Chromatography-Mass Spectrometry (UHPLC-MS)
2.8. Statistical Analysis
3. Results
3.1. Cardiac Lipid Metabolism is Altered in db/db Mouse Hearts with Further Changes Imparted by LAV-BPIFB4 Therapy
3.2. Changes in Cardiac Transcriptome of db/db Mice and Effect of LAV-BPIFB4 Therapy
3.3. Focused Expression Analysis of Mitochondrial-Related Pathways
4. Discussion
5. Patents
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Faulkner, A.; Dang, Z.; Avolio, E.; Thomas, A.C.; Batstone, T.; Lloyd, G.R.; Weber, R.J.; Najdekr, L.; Jankevics, A.; Dunn, W.B.; et al. Multi-Omics Analysis of Diabetic Heart Disease in the db/db Model Reveals Potential Targets for Treatment by a Longevity-Associated Gene. Cells 2020, 9, 1283. https://doi.org/10.3390/cells9051283
Faulkner A, Dang Z, Avolio E, Thomas AC, Batstone T, Lloyd GR, Weber RJ, Najdekr L, Jankevics A, Dunn WB, et al. Multi-Omics Analysis of Diabetic Heart Disease in the db/db Model Reveals Potential Targets for Treatment by a Longevity-Associated Gene. Cells. 2020; 9(5):1283. https://doi.org/10.3390/cells9051283
Chicago/Turabian StyleFaulkner, Ashton, Zexu Dang, Elisa Avolio, Anita C Thomas, Thomas Batstone, Gavin R Lloyd, Ralf JM Weber, Lukáš Najdekr, Andris Jankevics, Warwick B Dunn, and et al. 2020. "Multi-Omics Analysis of Diabetic Heart Disease in the db/db Model Reveals Potential Targets for Treatment by a Longevity-Associated Gene" Cells 9, no. 5: 1283. https://doi.org/10.3390/cells9051283