HIV Nef and Antiretroviral Therapy Have an Inhibitory Effect on Autophagy in Human Astrocytes that May Contribute to HIV-Associated Neurocognitive Disorders
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells and Treatments
2.2. Western Blotting
2.3. Fluorescence Microscopy
2.4. Transmission Electron Microscopy
2.5. qRT-PCR
2.6. Statistical Analysis
3. Results
3.1. Nef and/or ART Imbalance Autophagy
3.2. Nef Alone or in Combination with ART Accelerates APG Maturation in Astrocytes
3.3. Nef and/or ART Decrease the Number of APG in Human Astrocytes
3.4. Nef and/or ART induce “Long-Term” Autophagy Imbalance in Astrocytes
3.5. Nef and/or ART Compromise p62-Mediated Selective Autophagy Short and “Long Term”
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Cheney, L.; Guzik, H.; Macaluso, F.P.; Macian, F.; Cuervo, A.M.; Berman, J.W. HIV Nef and Antiretroviral Therapy Have an Inhibitory Effect on Autophagy in Human Astrocytes that May Contribute to HIV-Associated Neurocognitive Disorders. Cells 2020, 9, 1426. https://doi.org/10.3390/cells9061426
Cheney L, Guzik H, Macaluso FP, Macian F, Cuervo AM, Berman JW. HIV Nef and Antiretroviral Therapy Have an Inhibitory Effect on Autophagy in Human Astrocytes that May Contribute to HIV-Associated Neurocognitive Disorders. Cells. 2020; 9(6):1426. https://doi.org/10.3390/cells9061426
Chicago/Turabian StyleCheney, Laura, Hillary Guzik, Frank P. Macaluso, Fernando Macian, Ana Maria Cuervo, and Joan W. Berman. 2020. "HIV Nef and Antiretroviral Therapy Have an Inhibitory Effect on Autophagy in Human Astrocytes that May Contribute to HIV-Associated Neurocognitive Disorders" Cells 9, no. 6: 1426. https://doi.org/10.3390/cells9061426