Bromodomain Protein BRD4 Accelerates Glucocorticoid Dysregulation of Bone Mass and Marrow Adiposis by Modulating H3K9 and Foxp1
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bone-Marrow Mesenchymal Stem Cell Cultures
2.2. In Vitro Osteogenesis and Adipogenesis Assay
2.3. RNA Interference and cDNA Transfection
2.4. RT-PCR
2.5. Immunoblotting
2.6. Immunofluorescence
2.7. Chromatin Immunoprecipitation (ChIP)-PCR
2.8. Chromatin Immunoprecipitation-Sequencing (ChIP-seq)
2.9. Glucocorticoid-Induced Bone Loss in Mice
2.10. μCT Analysis
2.11. Ex Vivo Osteogenesis and Adipogenesis
2.12. Histomorphometry
2.13. Statistical Analysis
3. Results
3.1. H3K9 Hypomethylation Dysregulated Osteogenic and Adipogenic Differentiation
3.2. BRD4-Regulated Osteogenic and Adipogenic Differentiation
3.3. BRD4 Inhibition Changed H3K9ac Enrichment at Coding Region of Lipid Regulators
3.4. Foxp1 Mediated Glucocorticoid Upregulation of Adipocyte Formation
3.5. BRD4 Inhibitor Treatment Slowed Glucocorticoid-Induced Bone Loss
4. Discussion
Author Contributions
Funding
Conflicts of Interest
References
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Wang, F.-S.; Chen, Y.-S.; Ko, J.-Y.; Kuo, C.-W.; Ke, H.-J.; Hsieh, C.-K.; Wang, S.-Y.; Kuo, P.-C.; Jahr, H.; Lian, W.-S. Bromodomain Protein BRD4 Accelerates Glucocorticoid Dysregulation of Bone Mass and Marrow Adiposis by Modulating H3K9 and Foxp1. Cells 2020, 9, 1500. https://doi.org/10.3390/cells9061500
Wang F-S, Chen Y-S, Ko J-Y, Kuo C-W, Ke H-J, Hsieh C-K, Wang S-Y, Kuo P-C, Jahr H, Lian W-S. Bromodomain Protein BRD4 Accelerates Glucocorticoid Dysregulation of Bone Mass and Marrow Adiposis by Modulating H3K9 and Foxp1. Cells. 2020; 9(6):1500. https://doi.org/10.3390/cells9061500
Chicago/Turabian StyleWang, Feng-Sheng, Yu-Shan Chen, Jih-Yang Ko, Chung-Wen Kuo, Huei-Jing Ke, Chin-Kuei Hsieh, Shao-Yu Wang, Pei-Chen Kuo, Holger Jahr, and Wei-Shiung Lian. 2020. "Bromodomain Protein BRD4 Accelerates Glucocorticoid Dysregulation of Bone Mass and Marrow Adiposis by Modulating H3K9 and Foxp1" Cells 9, no. 6: 1500. https://doi.org/10.3390/cells9061500
APA StyleWang, F. -S., Chen, Y. -S., Ko, J. -Y., Kuo, C. -W., Ke, H. -J., Hsieh, C. -K., Wang, S. -Y., Kuo, P. -C., Jahr, H., & Lian, W. -S. (2020). Bromodomain Protein BRD4 Accelerates Glucocorticoid Dysregulation of Bone Mass and Marrow Adiposis by Modulating H3K9 and Foxp1. Cells, 9(6), 1500. https://doi.org/10.3390/cells9061500