Unravelling the Complex Duplication History of Deuterostome Glycerol Transporters
, François Chauvigné 3, Alba Ferré 3, Frank Nilsen 1, Per Gunnar Fjelldal 2, Joan Cerdà 3 and Roderick Nigel Finn 1,3,*Round 1
Reviewer 1 Report
The article is very well written. I have very few concerns as listed below.
Line 1 – Abstract – I guess it should to article
In the title “Unravelling an unexpectedly” – this looks inappropriate
Abstract
“Transmembrane glycerol transport is an ancient biophysical property that evolved in selected subfamilies of water channel (aquaporin) proteins.” - ancient biophysical property- need to change
“interrogation” – here evolution, analysis or study will be more appropriate. Use of interrogation has different meaning
“We uncover independent examples of gene translocations” – Need improvment
“The combined findings form 37 the novel basis for proposing a pandeuterostome glp gene nomenclature” – Authors can find better conclusion remark at the end of abstract
Introduction
“superfamily of transmembrane water-conducting channels, the aquaporins” - -Please make sure, AQP is not a superfamily, AQP belong to MIP superfamily.
“through evolutionary time” – can be removed
Results
AQP are known to transport many i=uncharged small solutes. In results or discussion better to address evolutionary aspect for solute specificity more particularly for solutes other than the glycerol.
Authors have not considered conserved motifs which defines solute specificity for instance NPA motifs and Ar/R selectivity filters.
Author Response
The article is very well written. I have very few concerns as listed below.
Line 1 – Abstract – I guess it should to article
Response: We have change the title as follows: “Unravelling the complex duplication history of deuterostome glycerol transporters”
In the title “Unravelling an unexpectedly” – this looks inappropriate
Abstract
“Transmembrane glycerol transport is an ancient biophysical property that evolved in selected subfamilies of water channel (aquaporin) proteins.” - ancient biophysical property- need to change
Response: The use of the word “ancient” is correct in this context
“interrogation” – here evolution, analysis or study will be more appropriate. Use of interrogation has different meaning
Response: The word “interrogation” has been changed to “analyses”
“We uncover independent examples of gene translocations” – Need improvement
Response: This sentence has been changed to: “We uncover separate examples of gene translocations …”
“The combined findings form the novel basis for proposing a pandeuterostome glp gene nomenclature” – Authors can find better conclusion remark at the end of abstract
We have altered the sentence as follows: “The combined findings resolve the origins and diversification of glps over >800 million years and thus form the novel basis for proposing a pandeuterostome glp gene nomenclature.”
Introduction
“superfamily of transmembrane water-conducting channels, the aquaporins” - -Please make sure, AQP is not a superfamily, AQP belong to MIP superfamily.
Response: In 1993 the use of the term “aquaporin” was coined to encompass the superfamily of water channel proteins, see Agre, P.; Sasaki, S.; Chrispeels, M.J. Aquaporins: a family of water channel proteins. Am. J. Physiol. 1993, 265, F461." Hence MIP is an outdated terminology. See also Finn, R.N.; Cerdà, J. Aquaporin. In Encyclopedia of Signaling Molecules. 2nd Edition, Choi, S. Ed.; Springer: New York, 2018; pp. 1-18.
“through evolutionary time” – can be removed
Response: removed
Results
AQP are known to transport many i=uncharged small solutes. In results or discussion better to address evolutionary aspect for solute specificity more particularly for solutes other than the glycerol.
Response: The aim of the study, as explained in the introduction was gene discovery, not solute specificity which would need to be addressed experimentally.
Authors have not considered conserved motifs which defines solute specificity for instance NPA motifs and Ar/R selectivity filters.
Response: This was beyond the scope of the current study, which focused on gene discovery as explained in the point above.
Reviewer 2 Report
The authors carry out an interesting evolutionary study in Deuterostomia and provide a broad overview over the evolution of aquaglyceroporin (Glps). They identify many new Glps in various species and characterise salmon GlPs in more detail including a determination of their transport function.
Major
The quality of the figures is low. Please increase the resolution.
Minor
It is confusing that the authors propose that they studied the function of Glp in paleotetraploid salmonids and refer to Fig. 4A and Supplementary Fig. 14, whereas the functional data are presented in Fig. 5. Please align.
Author Response
The authors carry out an interesting evolutionary study in Deuterostomia and provide a broad overview over the evolution of aquaglyceroporin (Glps). They identify many new Glps in various species and characterise salmon GlPs in more detail including a determination of their transport function.
Major
The quality of the figures is low. Please increase the resolution.
Response: We have requested to upload high-resolution figures
Minor
It is confusing that the authors propose that they studied the function of Glp in paleotetraploid salmonids and refer to Fig. 4A and Supplementary Fig. 14, whereas the functional data are presented in Fig. 5. Please align.
Response: This is now corrected as follows: “Functional Glp repertoires in paleotetraploid salmonids
