The Effects of PK11195 and Protoporphyrin IX Can Modulate Chronic Alcohol Intoxication in Rat Liver Mitochondria under the Opening of the Mitochondrial Permeability Transition Pore
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals and Treatment
2.2. Isolation of Rat Liver Mitochondria and the Outer Mitochondrial Membrane
2.3. Evaluation of Mitochondrial Functions
2.4. Sample Preparation
2.5. Electrophoresis and Immunoblotting of the Mitochondrial Proteins
2.6. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Control | Ethanol | Control | Ethanol | Control | Ethanol | |
---|---|---|---|---|---|---|
Pairs No | Body weight before treatment, g | Body weight before treatment, g | Body weight after treatment, g | Body weight after treatment, g | Liver weight, g | Liver weight, g |
Pair 1 | 165.0 | 165.0 | 346.0 | 316.0 | 10.3 | 11.7 |
Pair 2 | 173.0 | 178.0 | 334.0 | 322.0 | 10.6 | 11.4 |
Pair 3 | 169.0 | 170.0 | 338.0 | 326.0 | 10.9 | 11.8 |
Pair 4 | 163.0 | 160.0 | 343.0 | 306.0 | 10.4 | 11.4 |
mean | 167.5 ± 4.4 | 168.2 ± 7.7 | 340.2 ± 5.3 | 317.5 ± 8.7 | 10.5 ± 0.3 | 11.6 ± 0.2 |
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Baburina, Y.; Odinokova, I.; Krestinina, O. The Effects of PK11195 and Protoporphyrin IX Can Modulate Chronic Alcohol Intoxication in Rat Liver Mitochondria under the Opening of the Mitochondrial Permeability Transition Pore. Cells 2020, 9, 1774. https://doi.org/10.3390/cells9081774
Baburina Y, Odinokova I, Krestinina O. The Effects of PK11195 and Protoporphyrin IX Can Modulate Chronic Alcohol Intoxication in Rat Liver Mitochondria under the Opening of the Mitochondrial Permeability Transition Pore. Cells. 2020; 9(8):1774. https://doi.org/10.3390/cells9081774
Chicago/Turabian StyleBaburina, Yulia, Irina Odinokova, and Olga Krestinina. 2020. "The Effects of PK11195 and Protoporphyrin IX Can Modulate Chronic Alcohol Intoxication in Rat Liver Mitochondria under the Opening of the Mitochondrial Permeability Transition Pore" Cells 9, no. 8: 1774. https://doi.org/10.3390/cells9081774