Ischemia-Like Stress Conditions Stimulate Trophic Activities of Adipose-Derived Stromal/Stem Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells and Cell Culture
2.2. Ischemic Culture Conditions
2.3. Live/Dead Staining
2.4. Quantification of DNA
2.5. MTT Assay
2.6. Adipogenic Differentiation of Ischemia-Treated ASCs
2.7. Glucose and Lactate Determination
2.8. Assays of Cytokines
2.9. RNA Isolation and Quantitative Real-Time PCR (qRT-PCR) Analysis
2.10. Preparation of Conditioned Medium
2.11. Tube Formation Assay
2.12. Proliferation and Metabolic Activity of Fibroblasts
2.13. Fibroblast Migration Assay
2.14. Statistical Analysis
3. Results
3.1. Characterization of ASCs under Glucose/Oxygen Deprivation
3.2. Secretory Potential of ASCs under Glucose/Oxygen Deprivation
3.3. Regenerative Effects of Conditioned Medium from Glucose/Oxygen-Deprived ASCs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Bachmann, J.; Ehlert, E.; Becker, M.; Otto, C.; Radeloff, K.; Blunk, T.; Bauer-Kreisel, P. Ischemia-Like Stress Conditions Stimulate Trophic Activities of Adipose-Derived Stromal/Stem Cells. Cells 2020, 9, 1935. https://doi.org/10.3390/cells9091935
Bachmann J, Ehlert E, Becker M, Otto C, Radeloff K, Blunk T, Bauer-Kreisel P. Ischemia-Like Stress Conditions Stimulate Trophic Activities of Adipose-Derived Stromal/Stem Cells. Cells. 2020; 9(9):1935. https://doi.org/10.3390/cells9091935
Chicago/Turabian StyleBachmann, Julia, Elias Ehlert, Matthias Becker, Christoph Otto, Katrin Radeloff, Torsten Blunk, and Petra Bauer-Kreisel. 2020. "Ischemia-Like Stress Conditions Stimulate Trophic Activities of Adipose-Derived Stromal/Stem Cells" Cells 9, no. 9: 1935. https://doi.org/10.3390/cells9091935
APA StyleBachmann, J., Ehlert, E., Becker, M., Otto, C., Radeloff, K., Blunk, T., & Bauer-Kreisel, P. (2020). Ischemia-Like Stress Conditions Stimulate Trophic Activities of Adipose-Derived Stromal/Stem Cells. Cells, 9(9), 1935. https://doi.org/10.3390/cells9091935