Insights into Xylan Degradation and Haloalkaline Adaptation through Whole-Genome Analysis of Alkalitalea saponilacus, an Anaerobic Haloalkaliphilic Bacterium Capable of Secreting Novel Halostable Xylanase
Abstract
:1. Introduction
2. Materials and Methods
2.1. Concentration and Characterization of the Xylanase
2.2. Genome Sequencing, Annotation and Analysis Pipelines
3. Results and Discussion
3.1. Characteristics of Alkalitalea saponilacus Xylanase
3.2. Genome Features of Alkalitalea saponilacus
3.3. The Identified Xylan-Degrading Related Enzymes in Alkalitalea saponilacus
3.4. The Predicted Xylan Degradation Pathways in Alkalitalea saponilacus
3.5. The Genes Involved in Adaptation to Saline-Alkaline Conditions in Alkalitalea saponilacus
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Locus Tag | Product Name | GH |
---|---|---|
CDL62_17705 | Endo-β-1,4-xylanase (XynA) | GH10 |
CDL62_00085 | β-xylosidase | GH43 |
CDL62_06240 | β-xylosidase | GH43 |
CDL62_06275 | β-xylosidase | GH43 |
CDL62_06380 | β-xylosidase | GH43 |
CDL62_15875 | β-xylosidase | GH43 |
CDL62_02285 | β-xylosidase | GH43 |
CDL62_00095 | α-glucuronidase | GH67 |
CDL62_00195 | α-L-arabinofuranosidase | GH43 |
CDL62_00495 | α-L-arabinofuranosidase | GH43 |
CDL62_12950 | α-L-arabinofuranosidase | GH43 |
CDL62_00395 | α-L-arabinofuranosidase | GH51 |
Product Name | Locus Tag |
---|---|
L-glutamine synthesis | |
L-glutamine synthetase, GlnA | CDL62_11360 |
Choline/glycine/proline betaine transporter (BCCT family) | |
Choline/glycine/proline betaine transport protein | CDL62_17705 |
Na+/solute symporter | |
Na+/solute symporter (SSS family) | CDL62_09935 |
Na+/solute symporter (SSS family) | CDL62_06475 |
Na+/solute symporter (SSS family) | CDL62_14105 |
Na+/solute symporter (SSS family) | CDL62_11075 |
K+ transport systems, potassium uptake protein (Trk family) | |
Trk system potassium uptake protein, TrkA | CDL62_03510 |
Trk system potassium uptake protein, TrkH | CDL62_03515 |
Trk system potassium uptake protein, TrkA | CDL62_03555 |
Trk system potassium uptake protein, TrkH | CDL62_12070 |
Na+/H+ antiporter (NhaC family) | |
H+/Na+ antiporter (NhaC family) | CDL62_06020 |
Multisubunit Na+/H+ antiporter | |
Multisubunit Na+/H+ antiporter, MrpA subunit | CDL62_14320 |
Multisubunit Na+/H+ antiporter, MrpB subunit | CDL62_14325 |
Multisubunit Na+/H+ antiporter, MrpC subunit | CDL62_14330 |
Multisubunit Na+/H+ antiporter, MrpD subunit | CDL62_14335 |
Multisubunit Na+/H+ antiporter, MnhE subunit | CDL62_14340 |
Multisubunit Na+/H+ antiporter, MnhF subunit | CDL62_14345 |
Multisubunit Na+/H+ antiporter, MrpG subunit | CDL62_14350 |
Monovalent Cation/H+ antiporter (CPA family) | |
K+/H+ antiporter (CPA1 family) | CDL62_09425 |
K+/H+ antiporter (CPA1 family) | CDL62_00125 |
Na+/H+ antiporter (CPA2 family) | CDL62_00920 |
Na+/H+ antiporter (CPA2 family) | CDL62_05390 |
F0F1-ATP synthase | |
ATP synthase F1 subcomplex gamma subunit, AtpG | CDL62_07555 |
ATP synthase F1 subcomplex alpha subunit, AtpA | CDL62_07560 |
ATP synthase F1 subcomplex delta subunit, AtpH | CDL62_07565 |
ATP synthase F0 subcomplex B subunit, AtpF | CDL62_07570 |
ATP synthase F0 subcomplex C subunit, AtpE | CDL62_07575 |
ATP synthase F0 subcomplex A subunit, AtpB | CDL62_07580 |
ATP synthase F1 subcomplex epsilon subunit, AtpC | CDL62_07660 |
ATP synthase F1 subcomplex beta subunit, AtpD | CDL62_07665 |
H+-transporting two-sector ATPase (V-type ATP synthase) | |
V/A-type H+-transporting ATPase subunit E, AtpE | CDL62_11640 |
V/A-type H+-transporting ATPase subunit A, AtpA | CDL62_11650 |
V/A-type H+-transporting ATPase subunit B, AtpB | CDL62_11655 |
V/A-type H+-transporting ATPase subunit D, AtpD | CDL62_11660 |
V/A-type H+-transporting ATPase subunit I, AtpI | CDL62_11665 |
V/A-type H+-transporting ATPase subunit K, AtpK | CDL62_11670 |
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Liao, Z.; Holtzapple, M.; Yan, Y.; Wang, H.; Li, J.; Zhao, B. Insights into Xylan Degradation and Haloalkaline Adaptation through Whole-Genome Analysis of Alkalitalea saponilacus, an Anaerobic Haloalkaliphilic Bacterium Capable of Secreting Novel Halostable Xylanase. Genes 2019, 10, 1. https://doi.org/10.3390/genes10010001
Liao Z, Holtzapple M, Yan Y, Wang H, Li J, Zhao B. Insights into Xylan Degradation and Haloalkaline Adaptation through Whole-Genome Analysis of Alkalitalea saponilacus, an Anaerobic Haloalkaliphilic Bacterium Capable of Secreting Novel Halostable Xylanase. Genes. 2019; 10(1):1. https://doi.org/10.3390/genes10010001
Chicago/Turabian StyleLiao, Ziya, Mark Holtzapple, Yanchun Yan, Haisheng Wang, Jun Li, and Baisuo Zhao. 2019. "Insights into Xylan Degradation and Haloalkaline Adaptation through Whole-Genome Analysis of Alkalitalea saponilacus, an Anaerobic Haloalkaliphilic Bacterium Capable of Secreting Novel Halostable Xylanase" Genes 10, no. 1: 1. https://doi.org/10.3390/genes10010001