FLNC Expression Level Influences the Activity of TEAD-YAP/TAZ Signaling
Abstract
:1. Introduction
2. Material and Methods
2.1. Generation of Flnc Knock-Out Cell Line Using CRISPR/Cas9
2.2. Cell Culture
2.3. Western Blotting
2.4. Immunofluorescence
2.5. Gene Expression Analysis
2.6. Plasmid Construction and Flnc Overexpression
2.7. Scratch Assay
2.8. Proliferation and Migration Analyses
2.9. Luciferase Assay
2.10. Statistical Analysis
3. Results
3.1. Generation and Characterization of FlncKO Cell Line
3.2. FlncKO Myoblasts Exhibit Increased Proliferation Dynamic and Reduced Migration Ability
3.3. FlncKO Cells are Characterized by Reduced Ability of Myogenic Differentiation
3.4. Downregulation of FLNC in Myoblasts Promotes TEAD-YAP/TAZ Activation
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Knyazeva, A.; Khudiakov, A.; Vaz, R.; Muravyev, A.; Sukhareva, K.; Sejersen, T.; Kostareva, A. FLNC Expression Level Influences the Activity of TEAD-YAP/TAZ Signaling. Genes 2020, 11, 1343. https://doi.org/10.3390/genes11111343
Knyazeva A, Khudiakov A, Vaz R, Muravyev A, Sukhareva K, Sejersen T, Kostareva A. FLNC Expression Level Influences the Activity of TEAD-YAP/TAZ Signaling. Genes. 2020; 11(11):1343. https://doi.org/10.3390/genes11111343
Chicago/Turabian StyleKnyazeva, Anastasia, Aleksandr Khudiakov, Raquel Vaz, Aleksey Muravyev, Ksenia Sukhareva, Thomas Sejersen, and Anna Kostareva. 2020. "FLNC Expression Level Influences the Activity of TEAD-YAP/TAZ Signaling" Genes 11, no. 11: 1343. https://doi.org/10.3390/genes11111343
APA StyleKnyazeva, A., Khudiakov, A., Vaz, R., Muravyev, A., Sukhareva, K., Sejersen, T., & Kostareva, A. (2020). FLNC Expression Level Influences the Activity of TEAD-YAP/TAZ Signaling. Genes, 11(11), 1343. https://doi.org/10.3390/genes11111343