A Four-Methylated lncRNAs-Based Prognostic Signature for Hepatocellular Carcinoma
Abstract
:1. Introduction
2. Materials and Methods
2.1. Data and Sources
2.2. Identification of Differential Expressed lncRNAs with Altered Methylation Status in HCC
2.3. Establishment of Prognostic Predictive Model and ROC Curve
2.4. LncRNAs Survival Analysis
2.5. Use Prognostic Risk Score Model to Evaluate HCC in Cell Line
2.6. Statistical Analyses
3. Results
3.1. Identification of DELs with Altered lncRNAs Methylation Status in HCC
3.2. Construction of Four-MDELs HCC Prognostic Risk Score Model
3.3. Validation and Assessment of Four-MDELs HCC Signature Prognostic Risk Score Model
3.4. Comparisons with Other Models
3.5. Application of Our Model in HCC Cell Lines
4. Discussion and Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Name | Chr | Start | End |
---|---|---|---|
AC025016.1 | chr11 | 5,944,985 | 5,949,571 |
LINC01183 | chr5 | 127,802,442 | 127,802,442 |
LINC01269 | chr14 | 70,707,575 | 70,707,575 |
LINC01164 | chr10 | 131,773,701 | 131,773,701 |
OS | AUC (Our Model) | AUC (Wang’s Model) |
---|---|---|
1-year | 0.84 | 0.67 |
3-year | 0.75 | 0.59 |
5-year | 0.80 | 0.63 |
Characteristics | Patients | Univariate Analysis | Multivariate Analysis | ||
---|---|---|---|---|---|
HR 1 (95% CI) 2 | p–Value | HR (95% CI) | p–Value | ||
Gender (male/female) | 255/122 | 0.758 (0.498–1.154) | 0.196 | 0.763 (0.482–1.208) | 0.249 |
Age (≤ 65 vs. > 65) | 235/141 | 0.666 (0.441––1.007) | 0.054 | 0.729 (0.463–1.145) | 0.17 |
Tumor (T3–T4/T1–T2) | 94/280 | 1.794 (1.146–2.808) | 0.011 | 0.715 (0.086–5.919) | 0.756 |
Pathologic stage (III–IV/I–II) | 91/262 | 1.483 (0.906–2.427) | 0.117 | 0.744 (0.298–1.856) | 0.526 |
BMI 3 (≥ 25 vs. <25) | 161/180 | 0.906 (0.577–1.423) | 0.669 | ||
Adjacent_hepatic_tissue_inflammation (no/yes) | 119/120 | 1.128 (0.662–1.923) | 0.657 | ||
Virus 4 (no/yes) | 211/166 | 0.649 (0.428–0.985) | 0.042 | ||
Fibrosis (no/yes) | 76/142 | 0.813 (0.474–1.397) | 0.454 | ||
Radiation therapy (no/yes) | 243/4 | 2.127 (0.29–15.622) | 0.458 | ||
Relative_family_cancer_history (no/yes) | 212/114 | 1.795 (1.16–2.779) | 0.009 | ||
Pharmaceutical_therapy (no/yes) | 103/26 | 2.189 (0.951–5.04) | 0.066 | ||
New tumor event after initial treatment (no/yes) | 149/142 | 1.592 (0.877–2.89) | 0.126 | 0.654 (0.179–2.382) | 0.519 |
Person neoplasm cancer status (with tumor/tumor free) | 123/157 | 2.208 (1.224–3.983) | 0.009 | 2.816 (0.774–10.245) | 0.116 |
PI (High–risk/Low–risk) | 174/174 | 1.838 (1.177–2.87) | 0.007 | 2.469 (1.238–4.923) | 0.01 |
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Liao, L.-E.; Hu, D.-D.; Zheng, Y. A Four-Methylated lncRNAs-Based Prognostic Signature for Hepatocellular Carcinoma. Genes 2020, 11, 908. https://doi.org/10.3390/genes11080908
Liao L-E, Hu D-D, Zheng Y. A Four-Methylated lncRNAs-Based Prognostic Signature for Hepatocellular Carcinoma. Genes. 2020; 11(8):908. https://doi.org/10.3390/genes11080908
Chicago/Turabian StyleLiao, Le-En, Dan-Dan Hu, and Yun Zheng. 2020. "A Four-Methylated lncRNAs-Based Prognostic Signature for Hepatocellular Carcinoma" Genes 11, no. 8: 908. https://doi.org/10.3390/genes11080908