Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. cDNA Cloning and Analyses of Hemocyanin from Eriphia verrucosa
2.3. 3D-Model of E. verrucosa Hemocyanin Subunit
2.4. Phylogenetic Analysis
3. Results
3.1. cDNA Sequence of E. verrucosa Hemocyanin Subunit
3.2. Homology and Phylogenetic Analysis of E. verrucosa Hemocyanin Subunit
3.3. Structural Features of the E. verrucosa Hemocyanin Subunit 5
3.4. Glycosylation Sites in the E. verrucosa Hemocyanin Subunit 5
4. Discussion
4.1. Isolation, Structural, and Functional Properties of E. verrucosa Hemocyanin Subunit 5
4.2. Glycosylation of E. verrucosa Hemocyanin Subunit 5 and Possible Role in Immune Response
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Acknowledgments
Conflicts of Interest
References
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Primer | Sequence 5′–3′ | References |
---|---|---|
Degenerate primer F | GAACTTTTTTTTTGGGTTCATCATCAACTTAC | [22] |
Degenerate primer R | TGTGTTCTCTGAAGATGTTATCCATGTACTT | [22] |
Gene-specific primer F | AAGTACATGGATAACATCTTCAG | In this study |
Gene-specific primer R1 | TTATGCCGAACTCAGCTGTGAAGT | In this study |
Gene-specific primer R2 | AAGCATCACGTGAGCTGTGTTA | In this study |
3′ anchor-specific primer | GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTTV where V = A,C,G | 5′ and 3′ RACE Kit, 2nd Generation |
5′ anchor-specific primer | GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTTV where V = A,C,G | 5′ and 3′ RACE Kit, 2nd Generation |
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Todorovska, E.; Ivanov, M.; Radkova, M.; Dolashki, A.; Dolashka, P. Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca). Genes 2021, 12, 93. https://doi.org/10.3390/genes12010093
Todorovska E, Ivanov M, Radkova M, Dolashki A, Dolashka P. Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca). Genes. 2021; 12(1):93. https://doi.org/10.3390/genes12010093
Chicago/Turabian StyleTodorovska, Elena, Martin Ivanov, Mariana Radkova, Alexandar Dolashki, and Pavlina Dolashka. 2021. "Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca)" Genes 12, no. 1: 93. https://doi.org/10.3390/genes12010093