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Article
Peer-Review Record

Identification, Molecular Characterization, and Tissue Expression Profiles of Three Smad Genes from Water Buffalo (Bubalus bubalis)

Genes 2021, 12(10), 1536; https://doi.org/10.3390/genes12101536
by Jie Zhang †, Guangle Zhang † and Yongwang Miao *
Reviewer 1: Anonymous
Genes 2021, 12(10), 1536; https://doi.org/10.3390/genes12101536
Submission received: 11 August 2021 / Revised: 14 September 2021 / Accepted: 24 September 2021 / Published: 28 September 2021
(This article belongs to the Special Issue Animal Domestication and Breeding)

Round 1

Reviewer 1 Report

Introduction
I am not convinced that to improve the water buffalo reproductive efficiency the molecular studies are needed. I would argue that your aim should be adjusted in order to support your actual analysis.

LINE 40 - "Gene breeding or genome technology..." - I do not understand this sentence.


Conclusions
I recommend to highlight who your findings will support water buffalo breeding world-wide

Figure 4 should be a heatmap to better present the results.
Figure 6 should be larger 

Author Response

Please see the attachment

Author Response File: Author Response.docx

Reviewer 2 Report

Dear authors,

Line 1 – “Smads are involved in a variety of biological activities by mediating BMP signals.” – wrong sentence. No descriptions of used abbreviations.

Line 2 – Descript “CDSs” too.

Line 119 – add reference of using software

Line 120 – descript “AASs”

Line 140 – add country information of manufacturer of PCR mix.

Line 155 – isolated by RT-PCR – is it true?

Line 156 – How are you calculated length of amplicons in these gel-frames?

Line 165 – You are can write common color mark for all ABC parts

Line 190 – Text in figure too small. Shorter text mark (only species names) and increase it.

Line 207 – In figure all bootstraps are 0 according to legend. Is it true?

 

 

 

Regards,

Author Response

Please see the attachment.

Author Response File: Author Response.docx

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