Lower Fractions of TCF4 Transcripts Spanning over the CTG18.1 Trinucleotide Repeat in Human Corneal Endothelium
Abstract
:1. Introduction
2. Materials and Methods
3. Results
3.1. FECD Patients and Controls Characteristics
3.2. TCF4 Genotyping
3.3. TCF4 mRNA Expression by Digital Droplet PCR (ddPCR)
3.4. Differential TCF4 mRNA Expression in Corneal Endothelium
3.5. Comparison of TCF4 mRNA Expression in Individual Assays (A to E) in WBC and CE
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Custom TaqMan® Assay | Primer and Probe (5′ ⟶ 3′) |
---|---|
A | F 1: GTCTCTCTTTTTAAAGTCTCTTTCCTTGGAA R 2: TTGAGCCAGTAAAATGTCCA Probe: TGTGGCCATTTAAGATGTT |
B | F: GGGATGTAAACTCGAATAAATTTCAAAGTG R: TTGAGCCAGTAAAATGTCCA Probe: AGGCTTCAGATTGTAACTGAC |
C | F: TGAACGCCGCCTCGG R: TTGAGCCAGTAAAATGTCCAC Probe: TGCACGGAGAGCCC |
E | F: CCATTCGTTCCTTTGCTTTTTGCA R: CCCCAGGACCCTGAGCTA Probe: TTGAGCCAGTAAAATGTC |
Tissue | Group | Sex | Age (Years) | TCF4 Allele 1 | TCF4 Allele 2 | TCF4 Genotype | |
---|---|---|---|---|---|---|---|
KFUH9 | CE 1 | control | female | 78 | 12 | 25 | TCF4− |
KFUH12 | CE | control | female | 25 | 12 | 18 | TCF4− |
KFUH13 | CE | control | female | 56 | 12 | 18 | TCF4− |
KFUH15 | CE | control | male | 76 | 12 | 18 | TCF4− |
FUH18 | CE | FECD | male | 76 | 76 | 87 | TCF4+ |
FUH19 | CE | FECD | male | 85 | 22 | 75 | TCF4+ |
FUH20 | CE | FECD | male | 85 | 12 | 93 | TCF4+ |
FUH21 | CE | FECD | male | 71 | 12 | 71 | TCF4+ |
FUH22 | CE | FECD | female | 63 | 12 | 105 | TCF4+ |
FUB1 | WBC 2 | FECD | female | 62 | 12 | 95 | TCF4+ |
FUB2 | WBC | FECD | female | 81 | 15 | 93 | TCF4+ |
FUB3 | WBC | FECD | female | 69 | 81 | >81 | TCF4+ |
FUB4 | WBC | FECD | male | 59 | 17 | >125 | TCF4+ |
FUB5 | WBC | FECD | female | 76 | 37 | 97 | TCF4+ |
FUB7 | WBC | FECD | female | 84 | 20 | 52 | TCF4+ |
FUB8 | WBC | FECD | male | 75 | 12 | 95 | TCF4+ |
FUB9 | WBC | FECD | female | 73 | 19 | 85 | TCF4+ |
FUB10 | WBC | FECD | male | 62 | 27 | 78 | TCF4+ |
FUB11 | WBC | FECD | female | 72 | 27 | 91 | TCF4+ |
FUB12 | WBC | FECD | female | 84 | 12 | 67 | TCF4+ |
FUB13 | WBC | FECD | female | 84 | 18 | 96 | TCF4+ |
FUB14 | WBC | FECD | female | 76 | 19 | 83 | TCF4+ |
FUB15 | WBC | FECD | male | 79 | 17 | 103 | TCF4+ |
FUB16 | WBC | FECD | female | 59 | 19 | 98 | TCF4+ |
FUB17 | WBC | FECD | female | 65 | 29 | 101 | TCF4+ |
7753 | WBC | FECD | male | 70 | 13 | 13 | TCF4− |
8360 | WBC | FECD | female | 75 | 15 | 19 | TCF4− |
1494 | WBC | FECD | female | 78 | 12 | 15 | TCF4− |
9309 | WBC | FECD | male | 74 | 13 | 13 | TCF4− |
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Westin, I.M.; Viberg, A.; Byström, B.; Golovleva, I. Lower Fractions of TCF4 Transcripts Spanning over the CTG18.1 Trinucleotide Repeat in Human Corneal Endothelium. Genes 2021, 12, 2006. https://doi.org/10.3390/genes12122006
Westin IM, Viberg A, Byström B, Golovleva I. Lower Fractions of TCF4 Transcripts Spanning over the CTG18.1 Trinucleotide Repeat in Human Corneal Endothelium. Genes. 2021; 12(12):2006. https://doi.org/10.3390/genes12122006
Chicago/Turabian StyleWestin, Ida Maria, Andreas Viberg, Berit Byström, and Irina Golovleva. 2021. "Lower Fractions of TCF4 Transcripts Spanning over the CTG18.1 Trinucleotide Repeat in Human Corneal Endothelium" Genes 12, no. 12: 2006. https://doi.org/10.3390/genes12122006