CircVAMP3: A circRNA with a Role in Alveolar Rhabdomyosarcoma Cell Cycle Progression
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Cultures and Transfections
2.2. RNA Isolation and RNase-R Treatment
2.3. Nucleus-Cytoplasm Subcellular Fractionation
2.4. Western Blot
2.5. M6A CLIP
2.6. Flow Cytometric Analysis of Cell Cycle
2.7. RNA-Sequencing and Bioinformatic Analyses
3. Results
3.1. CircVAMP3 Is Highly Expressed in RH4 Cells
3.2. CircVAMP3 Is Located in the Cytoplasm and Has a Differential m6A Methylation with Respect to Its Linear Counterpart
3.3. CircVAMP3 Knock-Down Affects Proliferation-Related Pathways in ARMS Cells
4. Discussion and Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Rossi, F.; Centrón-Broco, A.; Dattilo, D.; Di Timoteo, G.; Guarnacci, M.; Colantoni, A.; Beltran Nebot, M.; Bozzoni, I. CircVAMP3: A circRNA with a Role in Alveolar Rhabdomyosarcoma Cell Cycle Progression. Genes 2021, 12, 985. https://doi.org/10.3390/genes12070985
Rossi F, Centrón-Broco A, Dattilo D, Di Timoteo G, Guarnacci M, Colantoni A, Beltran Nebot M, Bozzoni I. CircVAMP3: A circRNA with a Role in Alveolar Rhabdomyosarcoma Cell Cycle Progression. Genes. 2021; 12(7):985. https://doi.org/10.3390/genes12070985
Chicago/Turabian StyleRossi, Francesca, Alvaro Centrón-Broco, Dario Dattilo, Gaia Di Timoteo, Marco Guarnacci, Alessio Colantoni, Manuel Beltran Nebot, and Irene Bozzoni. 2021. "CircVAMP3: A circRNA with a Role in Alveolar Rhabdomyosarcoma Cell Cycle Progression" Genes 12, no. 7: 985. https://doi.org/10.3390/genes12070985