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Volume 13
Issue 7
10.3390/genes13071189
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Article
Peer-Review Record

Identification and Characterization Roles of Phytoene Synthase (PSY) Genes in Watermelon Development

Genes 2022, 13(7), 1189; https://doi.org/10.3390/genes13071189
by Xufeng Fang 1,2, Peng Gao 1,2, Feishi Luan 2,* and Shi Liu 1,*
Reviewer 1:
Lu-Sheng Hsieh
Genes 2022, 13(7), 1189; https://doi.org/10.3390/genes13071189
Submission received: 23 May 2022 / Revised: 15 June 2022 / Accepted: 28 June 2022 / Published: 1 July 2022
(This article belongs to the Section Plant Genetics and Genomics)

Round 1

Reviewer 1 Report

Although this manuscript provides valuable result, its format needs revised.

 

1. Page 1, line2: role should be capitalized.

 

2. Page 1, lines 5-8: Please provide the e-mails of the first and second authors.

 

3. Page 1, line 9: Typo: aythor’s.

 

4. Page 1, line 31: Reference format (Yuan et al., 2015) is incorrect, please use numeral system. Also correct reference format in all manuscript.

 

5. Page 1, line 45: ‘However’ should be lower case.

 

6. Page 3, line 98: Place 2.1 in front of Plant materials. Line 110 and line 120: place 2.2 and 2.3, respectively. Also correct format in all manuscript.

 

7. Page 6, line 276: E. coli cells harboring EB were…. It is a little bit confused. As I read in the main text (line 266), EB is an E. coli strain, right?

 

8. Page 10, Figure 7.

Subfigure a: mild suggestion, the authors already used days after pollination as the title of x-axis. Numbers only (10, 18, …) may be simplified and clear.

Subfigure b: typos, 10DPA, 18DPA,…etc.

Subfigure c: typos, 10DPA, 20DPA,…etc.

Subfigures e and l: Standard deviation is unseen, why? Is sample size one? In subfigure l, the authors use ‘leave’ instead of ‘leaf’ in subfigures d, f, h, g and i.

 

9. Figures 8 and 9 have the same issues as Figure 7. Please carefully revise them.

Author Response

Please see the attachment

Author Response File: Author Response.doc

Reviewer 2 Report

This is an interesting manuscript about the role of phytoene synthase (PSY) in the biosynthesis of carotenoid and flesh color formation in watermelon with the identification of three ClPSY genes through the watermelon genome and characterization of the complete protein-coding sequences (CDS). Also, a heterologous omplementation system was used to study the ClPSY gene family with regard to enzyme activity. The manuscript shows novel results, and the objectives are very clear. The results are well presented, and their interpretation is relevant.

Author Response

Thank you very much for your recognition of our manuscript and work. Thanks.

Author Response File: Author Response.docx

Round 2

Reviewer 1 Report

The authors addressed all my comments. The manuscript is acceptable! 

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