Identification of the Key Pathways and Genes Involved in the Wax Biosynthesis of the Chinese White Wax Scale Insect (Ericerus pela Chavannes) by Integrated Weighted Gene Coexpression Network Analysis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. High-Throughput Transcriptome Sequencing
2.3. Weighted Correlation Network Analysis
2.3.1. Data Input and Preprocessing
2.3.2. Gene Network Construction and Module Identification
2.3.3. Relating Modules with Wax Secretion Trait Data
2.3.4. Network Visualization
2.4. Selection of Hub Genes
2.5. Drawing of Figures
3. Results
3.1. Transcriptome Sequencing Results
3.2. Data Input, Cleaning, and Pre-Processing
3.3. Analysis of Network Topology
3.4. Network Construction and Module Detection
3.5. Selection of Hub Genes
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Ding, W.-F.; Ling, X.-F.; Lu, Q.; Wang, W.-W.; Zhang, X.; Feng, Y.; Chen, X.-M.; Chen, H. Identification of the Key Pathways and Genes Involved in the Wax Biosynthesis of the Chinese White Wax Scale Insect (Ericerus pela Chavannes) by Integrated Weighted Gene Coexpression Network Analysis. Genes 2022, 13, 1364. https://doi.org/10.3390/genes13081364
Ding W-F, Ling X-F, Lu Q, Wang W-W, Zhang X, Feng Y, Chen X-M, Chen H. Identification of the Key Pathways and Genes Involved in the Wax Biosynthesis of the Chinese White Wax Scale Insect (Ericerus pela Chavannes) by Integrated Weighted Gene Coexpression Network Analysis. Genes. 2022; 13(8):1364. https://doi.org/10.3390/genes13081364
Chicago/Turabian StyleDing, Wei-Feng, Xiao-Fei Ling, Qin Lu, Wei-Wei Wang, Xin Zhang, Ying Feng, Xiao-Ming Chen, and Hang Chen. 2022. "Identification of the Key Pathways and Genes Involved in the Wax Biosynthesis of the Chinese White Wax Scale Insect (Ericerus pela Chavannes) by Integrated Weighted Gene Coexpression Network Analysis" Genes 13, no. 8: 1364. https://doi.org/10.3390/genes13081364