CTCF and Its Partners: Shaper of 3D Genome during Development
Round 1
Reviewer 1 Report
This review describes the recent advancements with an emphasis on features of CTCF partners and will also discuss the specific functions of CTCF-associated complexes in chromatin structure modulation, which should help to uncover epigenetic mechanisms of relevant developmental processes. The manuscript is well structured and well discussed. However, some points should be checked and corrected before its acceptance in this journal.
Therefore, according to my comments, I recommended the publication of the paper after minor revision.
· The study's background should be clearly stated. Describe the introduction and review of the work (Please add more information).
· The MS English needs to be improved. The article's English must be carefully checked for grammatical errors.
Author Response
Dear Reviewer:
Thank you for your comments concerning our manuscript“CTCF and its partners: shaper of 3D genome during development”(genes-1834004). These comments are all valuable and very helpful for revising and improving our paper, as well as the important guiding significance to our researches. We have studied comments carefully and have made correction which we hope meet with approval.
All modified parts are marked in red in the paper. You can find my itemized responses below and my revisions/corrections in the resubmission.
We deeply appreciate your consideration of our manuscript. Thank you and best regards.
Author Response File: 
Author Response.docx
Reviewer 2 Report
In the manuscript "CTCF and its partners: shaper of 3D genome during development", written by Sun X, Zhang J and Cao C, the role of CTCF, acting in modelling the 3D structure of the chromatin, is presented. The manuscript in the first part describes the basic facts about 3D structure of the genome and new techniques used for detection of the molecules involved in their structure. In the following paragraphs the main partners of CTCF are presented: cohesins, different transcriptional factors which also have a role in loop formation and are important in different developmental processes and RNA partners. At the end, posttranslational modifications of CTCF are described. Conclusions are given together with perspectives, directions of investigations in the future.
The manuscript is well organized, systematic, presents a huge amount of data which are up to date.
Minor comments:
line 50: sentences reorganization: ...usually located inside the nucleus. Whereas....
line 74: sentence reorganization: invariant across...
line 77: sentence reorganization
line 97: in eukaryotic mitosis cells
line 102: extrudes – needs object
line 109: which support
line112: sentence reorganization
lines 113, 201, 252, 330: sentence should not begin with And
line 115: Fbn2 explanation
line 117: omit coma
line 132: sentence reorganization
line 134: sentence reorganization
line 152: MCF10A are not "normal" cells
line 160,293: a great number
line 163, 232, 296 375: after among object is needed
line 169: can WIZ be defined as a key regulator?
line 172: sites in the genome
line 176: sentence reorganization
line 178: sentence reorganization 3 times DNA loops in the same sentence
line 182: recruited
line 189: sentence reorganization
line 191: showed
line 199: what
line 218: sentence reorganization
line 234: loop intensity?
line 240: both,
line 263: on one hand,
line 274: explanation of what is BHLHE40
line 300: explain the unfolding state of nucleosomes
line 306: regulated
line 338: for DNA. Not through... sentence reorganization
line 343: which are
line 345: have been developed, sentence reorganization
line 348: interacted with
line 356: until
line 358: classical
line 374: sentence reorganization
line 376: stabilize
line 393: sentence reorganization
line 404: explanation of interlaminar activity
line 406: nucleolus
line 410: omit also
line 413: cMYC
line 414: specially
line 415: ...can activate transcription... better explanation
line 416: desumoylation
line 430: a substrate for LATS kinase
line 442: sentence reorganization, no need for questions , a great number
line 458: sentence reorganization
line 462: Nickerson... made the concept
line 470: on the genome
line 484: question
All references should have all the authors (some are at al.).
Author Response
Dear Reviewer:
Thank you for your comments concerning our manuscript“CTCF and its partners: shaper of 3D genome during development”(genes-1834004). These comments are all valuable and very helpful for revising and improving our paper, as well as the important guiding significance to our researches. We have studied comments carefully and have made correction which we hope meet with approval.
All modified parts are marked in red in the paper. You can find my itemized responses below and my revisions/corrections in the resubmission.
We deeply appreciate your consideration of our manuscript. Thank you and best regards.
Author Response File: Author Response.docx
