Temporal and Spatial Gene Expression Profile of Stroke Recovery Genes in Mice

Götz, Jan; Wieters, Frederique; Fritz, Veronika J.; Käsgen, Olivia; Kalantari, Aref; Fink, Gereon R.; Aswendt, Markus

doi:10.3390/genes14020454

Open AccessArticle

Temporal and Spatial Gene Expression Profile of Stroke Recovery Genes in Mice

by

Jan Götz

^1,2,

Frederique Wieters

^1,2,

Veronika J. Fritz

^1,2,

Olivia Käsgen

^1,2,

Aref Kalantari

^1,2,

Gereon R. Fink

^1,2,3

and

Markus Aswendt

^1,2,*

¹

Faculty of Medicine, University of Cologne, 50923 Cologne, Germany

²

Department of Neurology, University Hospital Cologne, 50931 Cologne, Germany

³

Cognitive Neuroscience, Institute of Neuroscience and Medicine (INM-3), Research Centre Juelich, 52425 Juelich, Germany

^*

Author to whom correspondence should be addressed.

Genes 2023, 14(2), 454; https://doi.org/10.3390/genes14020454

Submission received: 28 December 2022 / Revised: 6 February 2023 / Accepted: 7 February 2023 / Published: 9 February 2023

(This article belongs to the Special Issue Genomics of Stroke)

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Abstract

Stroke patients show some degree of spontaneous functional recovery, but this is not sufficient to prevent long-term disability. One promising approach is to characterize the dynamics of stroke recovery genes in the lesion and distant areas. We induced sensorimotor cortex lesions in adult C57BL/6J mice using photothrombosis and performed qPCR on selected brain areas at 14, 28, and 56 days post-stroke (P14-56). Based on the grid walk and rotating beam test, the mice were classified into two groups. The expression of cAMP pathway genes Adora2a, Pde10a, and Drd2, was higher in poor- compared to well-recovered mice in contralesional primary motor cortex (cl-MOp) at P14&56 and cl-thalamus (cl-TH), but lower in cl-striatum (cl-Str) at P14 and cl-primary somatosensory cortex (cl-SSp) at P28. Plasticity and axonal sprouting genes, Lingo1 and BDNF, were decreased in cl-MOp at P14 and cl-Str at P28 and increased in cl-SSp at P28 and cl-Str at P14, respectively. In the cl-TH, Lingo1 was increased, and BDNF decreased at P14. Atrx, also involved in axonal sprouting, was only increased in poor-recovered mice in cl-MOp at P28. The results underline the gene expression dynamics and spatial variability and challenge existing theories of restricted neural plasticity.

Keywords: behavior; recovery rate; grid walk; rotating beam test; qPCR; cAMP pathway

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MDPI and ACS Style

Götz, J.; Wieters, F.; Fritz, V.J.; Käsgen, O.; Kalantari, A.; Fink, G.R.; Aswendt, M. Temporal and Spatial Gene Expression Profile of Stroke Recovery Genes in Mice. Genes 2023, 14, 454. https://doi.org/10.3390/genes14020454

AMA Style

Götz J, Wieters F, Fritz VJ, Käsgen O, Kalantari A, Fink GR, Aswendt M. Temporal and Spatial Gene Expression Profile of Stroke Recovery Genes in Mice. Genes. 2023; 14(2):454. https://doi.org/10.3390/genes14020454

Chicago/Turabian Style

Götz, Jan, Frederique Wieters, Veronika J. Fritz, Olivia Käsgen, Aref Kalantari, Gereon R. Fink, and Markus Aswendt. 2023. "Temporal and Spatial Gene Expression Profile of Stroke Recovery Genes in Mice" Genes 14, no. 2: 454. https://doi.org/10.3390/genes14020454

APA Style

Götz, J., Wieters, F., Fritz, V. J., Käsgen, O., Kalantari, A., Fink, G. R., & Aswendt, M. (2023). Temporal and Spatial Gene Expression Profile of Stroke Recovery Genes in Mice. Genes, 14(2), 454. https://doi.org/10.3390/genes14020454

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Temporal and Spatial Gene Expression Profile of Stroke Recovery Genes in Mice

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