Illumina SBS Sequencing and DNBSEQ Perform Similarly for Single-Cell Transcriptomics
Highlights
- DNBSEQ exhibited mildly superior sequence quality compared to Illumina SBS, as evidenced by a higher Phred score and a greater number of genes mapping to the reference genome.
- However, these improvements did not translate into significant differences in the single-cell applications. There was no significant difference in the annotation of cells into different cell types, and both technologies yielded the same top differentially expressed genes between the experimental conditions.
- As we show that the quality between these two technologies is similar, researchers choosing a sequencing technology can give more weight to other external factors such as support, cost, and convenience.
- Competition is gradually driving down scRNA-seq reagent prices. Researchers being aware of different NGS alternatives will increase competition in the sequencing market and also help to reduce sequencing costs.
- This may open the doors to scRNAseq for researchers with limited budgets or allow researchers to add more samples using the same budget, enabling them to answer the same biological questions with a greater statistical power or explore new questions.
Abstract
Share and Cite
Bestard-Cuche, N.; Munro, D.A.D.; Beniazza, M.; Priller, J.; Williams, A.; Corsinotti, A. Illumina SBS Sequencing and DNBSEQ Perform Similarly for Single-Cell Transcriptomics. Genes 2024, 15, 1436. https://doi.org/10.3390/genes15111436
Bestard-Cuche N, Munro DAD, Beniazza M, Priller J, Williams A, Corsinotti A. Illumina SBS Sequencing and DNBSEQ Perform Similarly for Single-Cell Transcriptomics. Genes. 2024; 15(11):1436. https://doi.org/10.3390/genes15111436
Chicago/Turabian StyleBestard-Cuche, Nadine, David A. D. Munro, Meryam Beniazza, Josef Priller, Anna Williams, and Andrea Corsinotti. 2024. "Illumina SBS Sequencing and DNBSEQ Perform Similarly for Single-Cell Transcriptomics" Genes 15, no. 11: 1436. https://doi.org/10.3390/genes15111436
APA StyleBestard-Cuche, N., Munro, D. A. D., Beniazza, M., Priller, J., Williams, A., & Corsinotti, A. (2024). Illumina SBS Sequencing and DNBSEQ Perform Similarly for Single-Cell Transcriptomics. Genes, 15(11), 1436. https://doi.org/10.3390/genes15111436